Identification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattle

Página do item simplificado
dc.contributor.authorMuniz, Maria Malane Magalhães [UNESP]
dc.contributor.authorFonseca, Larissa Fernanda Simielli [UNESP]
dc.contributor.authordos Santos Silva, Danielly Beraldo [UNESP]
dc.contributor.authorde Oliveira, Hinayah Rojas
dc.contributor.authorBaldi, Fernando [UNESP]
dc.contributor.authorChardulo, Artur Loyola [UNESP]
dc.contributor.authorFerro, Jesus Aparecido [UNESP]
dc.contributor.authorCánovas, Angela
dc.contributor.authorde Albuquerque, Lucia Galvão [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Guelph
dc.contributor.institutionNational Council for Scientific and Technological Development (CNPq)
dc.date.accessioned2021-06-25T11:09:38Z
dc.date.available2021-06-25T11:09:38Z
dc.date.issued2021-03-01
dc.description.abstractThe Warner-Bratzler shear force (WBSF) and myofibrillar fragmentation index (MFI) are complementary methodologies used to measure beef tenderness. Longissimus thoracis samples from the 20 most extreme bulls (out of 80 bulls set) for WBSF (tender (n = 10) and tough (n = 10)) and MFI (high (n = 10) and low (n = 10)) traits were collected to perform transcriptomic analysis using RNA-Sequencing. All analysis were performed through CLC Genomics Workbench. A total of 39 and 27 transcripts for WBSF and MFI phenotypes were DE, respectively. The possible DE novel mRNA isoforms, for WBSF and MFI traits, are myosin encoders (e.g. MYL1 and MYL6). In addition, we identified potential mRNA isoforms related to genes affecting the speed fibers degradation during the meat aging process. The DE novel transcripts are transcripted by genes with biological functions related to oxidative process, energy production and striated muscle contraction. The results suggest that the identified mRNA isoforms could be used as potential candidate to select animals in order to improve meat tenderness.en
dc.description.affiliationSchool of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)
dc.description.affiliationCentre for Genetic Improvement of Livestock Department of Animal Biosciences University of Guelph
dc.description.affiliationNational Council for Scientific and Technological Development (CNPq)
dc.description.affiliationSão Paulo State University (Unesp) College of Veterinary and Animal Science
dc.description.affiliationUnespSchool of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)
dc.description.affiliationUnespSão Paulo State University (Unesp) College of Veterinary and Animal Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001.
dc.description.sponsorshipIdFAPESP: 11/21241-0
dc.description.sponsorshipIdFAPESP: 2009/16118-5
dc.description.sponsorshipIdFAPESP: 2017/02470-3
dc.description.sponsorshipIdFAPESP: 2017/10630-2
dc.description.sponsorshipIdFAPESP: 2018/11154-2
dc.description.sponsorshipIdFAPESP: 2018/20026-8
dc.identifierhttp://dx.doi.org/10.1016/j.meatsci.2020.108378
dc.identifier.citationMeat Science, v. 173.
dc.identifier.doi10.1016/j.meatsci.2020.108378
dc.identifier.issn0309-1740
dc.identifier.scopus2-s2.0-85098524499
dc.identifier.urihttp://hdl.handle.net/11449/208282
dc.language.isoeng
dc.relation.ispartofMeat Science
dc.sourceScopus
dc.subjectMeat maturation
dc.subjectMFI
dc.subjectNovel transcripts
dc.subjectRNA-Seq
dc.subjectWBSF
dc.titleIdentification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattleen
dc.typeResenha

Arquivos

Coleções

Artigos - Tecnologia - FCAV
Artigos - Zootecnia - FCAV