Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)

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dc.contributor.authorDuran, Bruno Oliveira Silva [UNESP]
dc.contributor.authorGóes, Guilherme Alcarás [UNESP]
dc.contributor.authorZanella, Bruna Tereza Thomazini [UNESP]
dc.contributor.authorFreire, Paula Paccielli [UNESP]
dc.contributor.authorValente, Jessica Silvino [UNESP]
dc.contributor.authorSalomão, Rondinelle Artur Simões
dc.contributor.authorFernandes, Ana [UNESP]
dc.contributor.authorMareco, Edson Assunção
dc.contributor.authorCarvalho, Robson Francisco [UNESP]
dc.contributor.authorDal-Pai-Silva, Maeli [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of West São Paulo (UNOESTE)
dc.date.accessioned2019-10-06T17:03:15Z
dc.date.available2019-10-06T17:03:15Z
dc.date.issued2019-12-01
dc.description.abstractThe postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth.en
dc.description.affiliationSão Paulo State University (UNESP) Institute of Biosciences Department of Morphology
dc.description.affiliationUniversity of West São Paulo (UNOESTE)
dc.description.affiliationSão Paulo State University (UNESP) Institute of Biosciences Department of Chemistry and Biochemistry
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Biosciences Department of Morphology
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Biosciences Department of Chemistry and Biochemistry
dc.identifierhttp://dx.doi.org/10.1038/s41598-019-38536-4
dc.identifier.citationScientific Reports, v. 9, n. 1, 2019.
dc.identifier.doi10.1038/s41598-019-38536-4
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85061759873
dc.identifier.urihttp://hdl.handle.net/11449/190129
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleAscorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)en
dc.typeArtigo
unesp.author.orcid0000-0002-4901-7714[9]

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