α-Lipoic acid-enrichment partially reverses tissue ascorbic acid depletion in pacu (Piaractus mesopotamicus) fed vitamin C-devoid diets

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dc.contributor.authorPark, Kwan Ha
dc.contributor.authorTerjesen, Bendik Fyhn
dc.contributor.authorTesser, Marcelo Borges [UNESP]
dc.contributor.authorPortella, Maria Célia [UNESP]
dc.contributor.authorDabrowski, Konrad
dc.contributor.institutionOhio State University
dc.contributor.institutionKunsan National University
dc.contributor.institutionInstitute of Aquaculture Research
dc.contributor.institutionCoE
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:02Z
dc.date.available2014-05-27T11:22:02Z
dc.date.issued2006-12-01
dc.description.abstractEffects of dietary α-lipoic acid (LA) and ascorbic acid (AA) on the growth, tissue vitamin C and tocopherol (vitamin E) levels, and malondialdehyde levels were examined in the tropical fish pacu, Piaractus mesopotamicus. Pacu juveniles were fed one of four casein-gelatin-based diets for 8 weeks: with 0.05% AA and 0.1% LA (+AA+LA), with AA and without LA (+AA-LA), without AA and with LA (-AA+LA), and without AA and LA (-AA-LA). When the fish received quantities of feed equal to 1.9-2.5% of its body weight, growth was not influenced, regardless of the presence of AA or LA throughout most of the experimental period. Growth was, however, slightly but significantly lower at week 8 in the AA-deficient/LA-supplemented group. An AA-deficient diet caused a highly significant reduction in both total AA and dehydroascorbic acid content in the liver and gill tissues. This reduction of tissue AA concentrations was reversed in a significant manner by LA (antioxidant-sparing effect). The 8-week-long vitamin C deprivation was sufficient to initiate the reduction in tissue ascorbic acid; however, total ascorbate in the liver of fish in the (-)AA/(+)LA group was 127.7±54.3 nmol g-1 tissue, whereas it was 28.6±26.3 nmol g-1 in the (-)AA/(-)LA group, a 4.4-fold difference. This mitigating effect of the addition of the endogenous antioxidant LA to the diet indicates that LA exerts a vitamin C-sparing effect in teleost fish that by far exceeds the phenomena demonstrated in non-scurvy-prone mammals. There was no difference among the different diet groups for vitamin E and malondialdehyde levels in the liver. These results suggest that LA is a potent substance for the prevention of AA deficiency in cultured fishes. The optimal dietary level of LA needs to be determined in the light of the slight reduction in body weight gain after 8 weeks of feeding in the absence of AA. © Springer Science+Business Media, Inc. 2006.en
dc.description.affiliationSchool of Natural Resources Ohio State University, Columbus, OH 43210
dc.description.affiliationDepartment of Aquatic Life Medicine College of Ocean Science and Technology Kunsan National University, San-68 Miryong-Dong, Kunsan City, Chonbuk
dc.description.affiliationAKVAFORSK Institute of Aquaculture Research, N-6600 Sunndalsøra
dc.description.affiliationAquaculture Protein Center CoE, 1432 Ås
dc.description.affiliationSão Paulo State University Aquaculture Center, 14870-000 Jaboticabal, SP
dc.description.affiliationUnespSão Paulo State University Aquaculture Center, 14870-000 Jaboticabal, SP
dc.format.extent329-338
dc.identifierhttp://dx.doi.org/10.1007/s10695-006-9110-9
dc.identifier.citationFish Physiology and Biochemistry, v. 32, n. 4, p. 329-338, 2006.
dc.identifier.doi10.1007/s10695-006-9110-9
dc.identifier.issn0920-1742
dc.identifier.lattes8511641760287071
dc.identifier.scopus2-s2.0-33750993220
dc.identifier.urihttp://hdl.handle.net/11449/69230
dc.language.isoeng
dc.relation.ispartofFish Physiology and Biochemistry
dc.relation.ispartofjcr1.735
dc.relation.ispartofsjr0,690
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAntioxidanten
dc.subjectCharaciformesen
dc.subjectDehydroascorbic aciden
dc.subjectPacuen
dc.subjectScurvy-proneen
dc.subjectTocopherolen
dc.subjectalpha tocopherolen
dc.subjectantioxidanten
dc.subjectascorbic aciden
dc.subjectcaseinen
dc.subjectdehydroascorbic aciden
dc.subjectgelatinen
dc.subjectmalonaldehydeen
dc.subjectthioctic aciden
dc.subjectanimal experimenten
dc.subjectanimal tissueen
dc.subjectantioxidant activityen
dc.subjectascorbic acid deficiencyen
dc.subjectcontrolled studyen
dc.subjectdiet supplementationen
dc.subjectfood compositionen
dc.subjectgillen
dc.subjectgrowth rateen
dc.subjectliver levelen
dc.subjectnonhumanen
dc.subjectscurvyen
dc.subjectteleosten
dc.subjecttissue levelen
dc.subjectvitamin supplementationen
dc.subjectweight gainen
dc.subjectMammaliaen
dc.subjectPiaractus mesopotamicusen
dc.subjectPiscesen
dc.subjectTeleosteien
dc.titleα-Lipoic acid-enrichment partially reverses tissue ascorbic acid depletion in pacu (Piaractus mesopotamicus) fed vitamin C-devoid dietsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes8511641760287071

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Jaboticabal - CAUNESP - Centro de Aquicultura da Unesp