Potential effects of the herbicide Diuron on mammary and urinary bladder two-stage carcinogenesis in a female Swiss mouse model

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dc.contributor.authorde Moura, Nelci Antunes [UNESP]
dc.contributor.authorGrassi, Tony Fernando [UNESP]
dc.contributor.authorRodrigues, Maria Aparecida Marchesan [UNESP]
dc.contributor.authorBarbisan, Luis Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:25Z
dc.date.available2014-05-20T13:37:25Z
dc.date.issued2010-02-01
dc.description.abstractThe potential promoting effect of Diuron was investigated in a mouse model of mammary and urinary bladder carcinogenesis induced by 7,12-dimethylbenz(a)anthracene (DMBA) and N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). Four-week old female Swiss mice were allocated to five groups: Groups G1-G3 received DMBA (5 x 1.5 mg/mouse) and BBN (8 x 7.5 mg/mouse) and G4 and G5 groups received only vehicles during the first 6 weeks. At week 7, G1 and G5 groups received basal diet and G2, G3 and G4 groups were fed a diet containing Diuron at 1,250, 2,500 and 2,500 ppm, respectively, during 13 weeks. At week 20, the animals were euthanized and the gross tumors were registered. Mammary glands and urinary bladder were processed for histopathological analysis. Samples from non-tumor areas were evaluated for cell proliferation by 5-bromodeoxyuridine labeling index (BrdU-LI%) and apoptosis. Dietary treatment with Diuron at 1,250 and 2,500 ppm significantly increased BrdU-LI% (P < 0.05) and the incidence of simple/nodular urothelial hyperplasia in the urinary bladder from DMBA/BBN-initiated groups (G2 and G3 vs. G1, P < 0.02) and in the non-initiated group (G4 vs. G5, P = 0.042). Two transitional cell carcinomas were observed in the group initiated and fed Diuron 2,500 ppm (G3). In contrast, in the mammary gland, Diuron feeding for 13 weeks did not significantly alter cell proliferation and apoptosis indexes or the incidence of hyperplastic lesions or neoplasms in the DMBA/BBN-initiated groups. These findings suggest that Diuron is a promoting agent to the urinary bladder but not to the mammary gland in female Swiss mice submitted to a medium-term two-stage carcinogenesis bioassay.en
dc.description.affiliationUNESP São Paulo State Univ, Dept Morphol, Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNESP São Paulo State Univ, Dept Pathol, Fac Med, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Morphol, Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Pathol, Fac Med, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/58937-7
dc.description.sponsorshipIdFAPESP: 06/01330-0
dc.format.extent165-173
dc.identifierhttp://dx.doi.org/10.1007/s00204-009-0477-0
dc.identifier.citationArchives of Toxicology. New York: Springer, v. 84, n. 2, p. 165-173, 2010.
dc.identifier.doi10.1007/s00204-009-0477-0
dc.identifier.issn0340-5761
dc.identifier.lattes3278528112652257
dc.identifier.urihttp://hdl.handle.net/11449/12940
dc.identifier.wosWOS:000274288600010
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofArchives of Toxicology
dc.relation.ispartofjcr5.728
dc.relation.ispartofsjr1,541
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPesticidesen
dc.subjectDiuronen
dc.subjectMammary and urinary bladder carcinogenesisen
dc.subjectFemale Swiss mouseen
dc.titlePotential effects of the herbicide Diuron on mammary and urinary bladder two-stage carcinogenesis in a female Swiss mouse modelen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes3278528112652257
unesp.author.orcid0000-0002-2718-1564[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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