Peri-ovulatory endocrine regulation of the prostanoid pathways in the bovine uterus at early dioestrus

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dc.contributor.authorOliveira, Milena Lopes
dc.contributor.authorD'Alexandri, Fabio Luiz
dc.contributor.authorPugliesi, Guilherme
dc.contributor.authorVan Hoeck, Veerle
dc.contributor.authorMesquita, Fernando Silveira
dc.contributor.authorMembrive, Claudia M. B. [UNESP]
dc.contributor.authorNegrao, Joao Alberto
dc.contributor.authorWheelock, Craig E.
dc.contributor.authorBinelli, Mario
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Fed Pampa
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionKarolinska Inst Stockholm
dc.date.accessioned2018-11-28T04:17:16Z
dc.date.available2018-11-28T04:17:16Z
dc.date.issued2017-01-01
dc.description.abstractWe hypothesised that different endocrine profiles associated with pre-ovulatory follicle (POF) size would impact on uterine prostanoid pathways and thereby modulate the histotroph composition. Beef cows (n=15 per group) were hormonally manipulated to have small (SF-SCL group) or large (LF-LCL group) pre-ovulatory follicles (POF) and corpora lutea (CL). Seven days after induction of ovulation, animals were slaughtered and uterine tissues and flushings were collected for quantification of prostanoids. The POF and CL size and the circulating progesterone concentrations at Day 7 were greater (P<0.05) in the LF-LCL cows than in the SF-SCL group, as expected. The abundance of 5 out of 19 genes involved in prostanoid regulation was different between groups. Transcript abundance of prostaglandin F2, E2 and I2 synthases was upregulated (P<0.05) and phospholipase A2 was downregulated (P<0.05) in endometrium of the LF-LCL group. No difference (P>0.1) in prostanoid concentrations in the endometrium or in uterine flushings was detected between groups. However, prostaglandin F2 and E2 concentrations in the uterine flushings were positively correlated with the abundance of transcripts for prostaglandin endoperoxide synthase 2 (0.779 and 0.865, respectively; P<0.002). We conclude that endometrial gene expression related to prostanoid synthesis is modulated by the peri-ovulatory endocrine profile associated with POF size, but at early dioestrus differences in transcript abundance were not reflected in changes in prostanoid concentrations in the uterine tissue and fluid.en
dc.description.affiliationUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Ave Duque de Caxias Norte 225, BR-13630000 Pirassununga, SP, Brazil
dc.description.affiliationUniv Fed Pampa, Sch Vet Med, BR472,Km592, BR-97508000 Uruguaiana, RS, Brazil
dc.description.affiliationUniv Estadual Paulista, Rodovia Comandante Joao Ribeiro de Barros,Km65, BR-17900000 Dracena, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Coll Anim Sci & Food Engn, Ave Duque de Caxias Norte 225, BR-13630000 Pirassununga, SP, Brazil
dc.description.affiliationKarolinska Inst Stockholm, Div Physiol Chem 2, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden
dc.description.affiliationUnespUniv Estadual Paulista, Rodovia Comandante Joao Ribeiro de Barros,Km65, BR-17900000 Dracena, SP, Brazil
dc.description.sponsorshipLFEM
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdLFEM: 204
dc.description.sponsorshipIdLFEM: 206
dc.description.sponsorshipIdCNPq: 481199/2012-8
dc.description.sponsorshipIdFAPESP: 2011/03226-4
dc.format.extent544-556
dc.identifierhttp://dx.doi.org/10.1071/RD15269
dc.identifier.citationReproduction Fertility And Development. Clayton: Csiro Publishing, v. 29, n. 3, p. 544-556, 2017.
dc.identifier.doi10.1071/RD15269
dc.identifier.fileWOS000395555500011.pdf
dc.identifier.issn1031-3613
dc.identifier.lattes5720162493397851
dc.identifier.orcid0000-0003-4211-0649
dc.identifier.urihttp://hdl.handle.net/11449/165492
dc.identifier.wosWOS:000395555500011
dc.language.isoeng
dc.publisherCsiro Publishing
dc.relation.ispartofReproduction Fertility And Development
dc.relation.ispartofsjr0,681
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectendometrium
dc.subjectoestrogen
dc.subjectphysiology
dc.subjectprostaglandins
dc.titlePeri-ovulatory endocrine regulation of the prostanoid pathways in the bovine uterus at early dioestrusen
dc.typeArtigo
dcterms.rightsHolderCsiro Publishing
unesp.author.lattes5720162493397851[6]
unesp.author.orcid0000-0003-4211-0649[6]

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