Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design

Página do item simplificado
dc.contributor.authorGiese, Ellen C. [UNESP]
dc.contributor.authorDekker, Robert F.H.
dc.contributor.authorScarminio, Ieda S.
dc.contributor.authorBarbosa, Aneli M.
dc.contributor.authorda Silva, Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionLakehead University
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2014-05-27T11:25:27Z
dc.date.available2014-05-27T11:25:27Z
dc.date.issued2011-01-15
dc.description.abstractBotryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its consumption (after 108h), and de-repression of β-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of β-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of β-1,3-glucanases and β-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used to optimize β-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30UmL -1), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for β-glucanase production by T. harzianum (4.25UmL -1). © 2010 Elsevier B.V.en
dc.description.affiliationDepartamento de Química e Ciências Ambientais Universidade Estadual Paulista São José do Rio Preto-SP
dc.description.affiliationBiorefining Research Initiative Lakehead University, Thunder Bay-ON
dc.description.affiliationDepartamento de Química Universidade Estadual de Londrina, Londrina-PR
dc.description.affiliationDepartamento de Bioquímica e Biotecnologia Universidade Estadual de Londrina, Londrina-PR
dc.description.affiliationUnespDepartamento de Química e Ciências Ambientais Universidade Estadual Paulista São José do Rio Preto-SP
dc.format.extent239-243
dc.identifierhttp://dx.doi.org/10.1016/j.bej.2010.10.013
dc.identifier.citationBiochemical Engineering Journal, v. 53, n. 2, p. 239-243, 2011.
dc.identifier.doi10.1016/j.bej.2010.10.013
dc.identifier.file2-s2.0-78649960162.pdf
dc.identifier.issn1369-703X
dc.identifier.lattes9424175688206545
dc.identifier.scopus2-s2.0-78649960162
dc.identifier.urihttp://hdl.handle.net/11449/72299
dc.language.isoeng
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.ispartofjcr3.226
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectβ-1,3-Glucanases
dc.subjectBotryosphaeran
dc.subjectStatistical mixture-design optimization
dc.subjectBotryosphaeria rhodina MAMB-05
dc.subjectCarbon source
dc.subjectCurdlan
dc.subjectDe-repression
dc.subjectEnzyme production
dc.subjectExponential growth
dc.subjectGlucanase
dc.subjectLaminarins
dc.subjectNutrient medium
dc.subjectSynergic effects
dc.subjectTrichoderma harzianum Rifai
dc.subjectDesign
dc.subjectEnzymes
dc.subjectFungi
dc.subjectGlucose
dc.subjectMixtures
dc.subjectOptimization
dc.subject1,3 beta glucanase
dc.subjectcurdlan
dc.subjectglucose
dc.subjectlactose
dc.subjectlaminaran
dc.subjectbotryosphaeria rhodina
dc.subjectcomparative study
dc.subjectcorrelation analysis
dc.subjectenzyme substrate
dc.subjectenzyme synthesis
dc.subjectfungus
dc.subjectHypocrea lixii
dc.subjectmycelium
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectstatistical analysis
dc.subjectBotryosphaeria rhodina
dc.titleComparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-designen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes9424175688206545
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-78649960162.pdf
Tamanho:
610.52 KB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Química e Ciências Ambientais - IBILCE