Morphology and protein content of hepatocytes in type I diabetic rats submitted to physical exercises

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dc.contributor.authorRemedio, Rafael N. [UNESP]
dc.contributor.authorCastellar, Alexandre [UNESP]
dc.contributor.authorBarbosa, Rodrigo A. [UNESP]
dc.contributor.authorGomes, Ricardo J.
dc.contributor.authorCaetano, Flávio Henrique [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-20T13:55:11Z
dc.date.available2014-05-20T13:55:11Z
dc.date.issued2011-07-01
dc.description.abstractThe importance of physical exercise practice in the treatment of diabetes has been reported in many studies recently, but only limited data can be found regarding its benefits on liver morphology and protein content of hepatocytes. In order to assess the changes arising from the development of type I diabetes and the benefits of a training protocol, Wistar rats were divided into four groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). The training protocol consisted of swimming for 60 min a day, 5 days/week, during 8 weeks. Liver samples were collected, processed and analyzed by histochemical and ultrastructural techniques. Biochemical tests were also conducted to examine the protein content and quantity of DNA in the liver. In morphological assessment, the presence of areas of cytoplasmic basophilia observed in control subjects was not visualized in sedentary diabetics. It was related to differences in the amount of mitochondria in the cytosol. The mitochondrial structure has not undergone relevant changes, and the number of rough endoplasmic reticulum cisterns was clearly inferior in sedentary diabetics, suggesting lower protein production. However, the biochemical analysis of protein content indicated no statistical differences between groups. The exercise, in turn, was not responsible for major changes in these characteristics. on the whole, the morphological damages arising from type I diabetes were noteworthy. Nevertheless, regular physical training was not responsible for significant improvements in some respects, making evident the need for combined application of a distinct form of treatment. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.description.affiliationSão Paulo State Univ UNESP, Dept Biol, Rio Claro, SP, Brazil
dc.description.affiliationSão Paulo Fed Univ UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Biol, Rio Claro, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent484-491
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2011.01.008
dc.identifier.citationMicron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 42, n. 5, p. 484-491, 2011.
dc.identifier.doi10.1016/j.micron.2011.01.008
dc.identifier.issn0968-4328
dc.identifier.lattes4396826019535898
dc.identifier.urihttp://hdl.handle.net/11449/19743
dc.identifier.wosWOS:000289322900015
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMicron
dc.relation.ispartofjcr1.728
dc.relation.ispartofsjr0,624
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDiabetesen
dc.subjectExerciseen
dc.subjectLiveren
dc.subjectUltrastructureen
dc.subjectHistologyen
dc.subjectProteinen
dc.titleMorphology and protein content of hepatocytes in type I diabetic rats submitted to physical exercisesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes4396826019535898
unesp.author.orcid0000-0002-9913-8565[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC