PDGF-B gene therapy accelerates bone engineering and oral implant osseointegration

Página do item simplificado
dc.contributor.authorChang, P-C
dc.contributor.authorSeol, Y-J
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.authorPellegrini, G.
dc.contributor.authorJin, Q.
dc.contributor.authorFranco, L. M.
dc.contributor.authorGoldstein, S. A.
dc.contributor.authorChandler, L. A.
dc.contributor.authorSosnowski, B.
dc.contributor.authorGiannobile, W. V.
dc.contributor.institutionUniversity of Michigan
dc.contributor.institutionSeoul Natl Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Milan
dc.contributor.institutionTissue Repair Co
dc.date.accessioned2013-09-30T18:32:01Z
dc.date.accessioned2014-05-20T13:45:31Z
dc.date.available2013-09-30T18:32:01Z
dc.date.available2014-05-20T13:45:31Z
dc.date.issued2010-01-01
dc.description.abstractPlatelet-derived growth factor-BB (PDGF-BB) stimulates repair of healing-impaired chronic wounds such as diabetic ulcers and periodontal lesions. However, limitations in predictability of tissue regeneration occur due, in part, to transient growth factor bioavailability in vivo. Here, we report that gene delivery of PDGF-B stimulates repair of oral implant extraction socket defects. Alveolar ridge defects were created in rats and were treated at the time of titanium implant installation with a collagen matrix containing an adenoviral (Ad) vector encoding PDGF-B (5.5 x 10(8) or 5.5 x 10(9) pfu ml (1)), Ad encoding luciferase (Ad-Luc; 5.5 x 10(9) pfu ml (1); control) or recombinant human PDGF-BB protein (rhPDGF-BB, 0.3 mg ml (1)). Bone repair and osseointegration were measured through backscattered scanning electron microscopy, histomorphometry, microcomputed tomography and biomechanical assessments. Furthermore, a panel of local and systemic safety assessments was performed. Results indicated that bone repair was accelerated by Ad-PDGF-B and rhPDGF-BB delivery compared with Ad-Luc, with the high dose of Ad-PDGF-B more effective than the low dose. No significant dissemination of the vector construct or alteration of systemic parameters was noted. In summary, gene delivery of Ad-PDGF-B shows regenerative and safety capabilities for bone tissue engineering and osseointegration in alveolar bone defects comparable with rhPDGF-BB protein delivery in vivo. Gene Therapy (2010) 17, 95-104; doi: 10.1038/gt.2009.117; published online 10 September 2009en
dc.description.affiliationUniv Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
dc.description.affiliationUniv Michigan, Coll Engn, Dept Biomed Engn, Ann Arbor, MI 48109 USA
dc.description.affiliationSeoul Natl Univ, Sch Dent, Dept Periodontol, Seoul, South Korea
dc.description.affiliationState Univ São Paulo, Sch Dent Araraquara, Dept Periodontol, São Paulo, Brazil
dc.description.affiliationUniv Milan, Clin Hosp Mangiagalli, Dept Periodontol, Milan, Italy
dc.description.affiliationUniv Michigan, Sch Med, Dept Orthopaed Surg, Ann Arbor, MI 48109 USA
dc.description.affiliationTissue Repair Co, San Diego, CA USA
dc.description.affiliationUnespState Univ São Paulo, Sch Dent Araraquara, Dept Periodontol, São Paulo, Brazil
dc.description.sponsorshipNational Institutes of Health (NIH)/National Institute of Dental and Craniofacial Research (NIDCR)
dc.description.sponsorshipAO Foundation Research Advisory Council
dc.description.sponsorshipIdNIH: R01-DE13397
dc.format.extent95-104
dc.identifierhttp://dx.doi.org/10.1038/gt.2009.117
dc.identifier.citationGene Therapy. London: Nature Publishing Group, v. 17, n. 1, p. 95-104, 2010.
dc.identifier.doi10.1038/gt.2009.117
dc.identifier.issn0969-7128
dc.identifier.lattes2628593693450121
dc.identifier.urihttp://hdl.handle.net/11449/16018
dc.identifier.wosWOS:000273408200010
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofGene Therapy
dc.relation.ispartofjcr3.203
dc.relation.ispartofsjr1,277
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdental implanten
dc.subjectplatelet-derived growth factoren
dc.subjectregenerative medicineen
dc.subjectvirus deliveryen
dc.titlePDGF-B gene therapy accelerates bone engineering and oral implant osseointegrationen
dc.typeArtigo
dcterms.licensehttp://www.nature.com/authors/policies/confidentiality.html
dcterms.rightsHolderNature Publishing Group
unesp.author.lattes2628593693450121
unesp.author.orcid0000-0002-2076-5452[2]
unesp.author.orcid0000-0002-4493-6922[1]
unesp.author.orcid0000-0002-7102-9746[10]
unesp.author.orcid0000-0002-7082-9290[3]
unesp.author.orcid0000-0002-4179-7066[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Diagnóstico e Cirurgia - FOAR