Purification and characterization of a cyclomaltodextrin glucanotransferase from Paenibacillus campinasensis strain H69-3

Página do item simplificado
dc.contributor.authorAlves-Prado, Heloiza Ferreira
dc.contributor.authorGomes, Eleni
dc.contributor.authorda Silva, Roberto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:56Z
dc.date.available2014-05-20T15:27:56Z
dc.date.issued2007-03-01
dc.description.abstractA cyclomaltodextrin glucanotransferase (E.C. 2.4.1.19) from a newly isolated alkalophilic and moderately thermophilic Paenibacillus campinasensis strain H69-3 was purified as a homogeneous protein from culture supernatant. Cyclomaltodextrin glucanotransferase was produced during submerged fermentation at 45 degrees C and purified by gel filtration on Sephadex G50 ion exchange using a Q-Sepharose column and ion exchange using a Mono-Q column. The molecular weight of the purified enzyme was 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the pI was 5.3. The optimum pH for enzyme activity was 6.5, and it was stable in the pH range 6.0-11.5. The optimum temperature was 65 degrees C at pH 6.5, and it was thermally stable up to 60 degrees C without substrate during 1 h in the presence of 10 mm CaCl2. The enzyme activity increased in the presence of Co2+, Ba2+, and Mn2+. Using maltodextrin as substrate, the K-m and K-cat were 1.65 mg/mL and 347.9 mu mol/mg.min, respectively.en
dc.description.affiliationUNESP, Biochem & Appl Microbiol Lab, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUNESP, Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Biochem & Appl Microbiol Lab, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Rio Claro, SP, Brazil
dc.format.extent41-55
dc.identifierhttp://dx.doi.org/10.1007/s12010-007-9038-2
dc.identifier.citationApplied Biochemistry and Biotechnology. Totowa: Humana Press Inc., v. 137, p. 41-55, 2007.
dc.identifier.doi10.1007/s12010-007-9038-2
dc.identifier.issn0273-2289
dc.identifier.lattes7091241742851920
dc.identifier.lattes9424175688206545
dc.identifier.lattes0000-0003-0935-1387
dc.identifier.urihttp://hdl.handle.net/11449/37844
dc.identifier.wosWOS:000246616900006
dc.language.isoeng
dc.publisherHumana Press Inc
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.ispartofjcr1.797
dc.relation.ispartofsjr0,571
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCGTase characterizationpt
dc.subjectCGTase purificationpt
dc.subjectcyclomaltodextrin glucanotransferasept
dc.subjectthermostable CGTasept
dc.titlePurification and characterization of a cyclomaltodextrin glucanotransferase from Paenibacillus campinasensis strain H69-3en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderHumana Press Inc
unesp.author.lattes7091241742851920[2]
unesp.author.lattes9424175688206545
unesp.author.orcid0000-0003-0935-1387[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biologia - IBILCE