CYTOMETRY AND KARYOMETRY IN TRYPANOSOMA-CRUZI

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dc.contributor.authorRabenhorst, S. H.
dc.contributor.authorSogayar, R.
dc.contributor.authorGuimaraes, R. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:29:45Z
dc.date.available2014-05-20T15:29:45Z
dc.date.issued1993-09-01
dc.description.abstractA total of 991 Trypanosoma cruzi cells, from four laboratory stocks, including the three differentiation forms, had their cellular outlines, nuclei and kinetoplasts measured at 9000 x magnification. Data on the identifiable cell cycle stages were used to search for intraspecific and biological cycle heterogeneity.Cellular areas (CA) in the interphasic differentiation forms produced ratios of 1.07 for culture epimastigotes (E), 1 for blood trypomastigotes (T), and 0.86 for tissue forms (A). Homogeneity in terms of nuclear (NA) and kinetoplast (KA) areas prevailed among the stocks, with differences of at most 6%, for modal NA of strains CL and Y. NA of T-form was larger than the basic NA of early G1 A-form. T-form kinetoplast volume was 3-fold that of A-form K-DNA nucleoids.One of the two recently divided kinetoplasts in mitotic E-form did not correlate with CA, indicating that mitochondrial division was unequal. The KA of CL strain T-form did not correlate with NA, suggesting a mitochondrial disfunction in this thermosensitive strain.The CL strain T-form was more heterogeneous than the Y strain for all characters, showing greater frequency of large values, even reaching the G2 levels. This heterogeneity was interpreted as functional, consequent to the thermosensitivity of the CL strain. Precocious bursting of CL strain host cells would lead to the polymorphic T-forms. Post-S phase trypomastigotes could start division soon after penetration of host cells.en
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO, INST BIOCIENCIAS, DEPT GENET, BR-18618 BOTUCATU, SP, BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO, INST BIOCIENCIAS, DEPT GENET, BR-18618 BOTUCATU, SP, BRAZIL
dc.format.extent521-535
dc.identifierhttp://www.gmb.org.br/Revistas/V16/v16a046.pdf
dc.identifier.citationRevista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 3, p. 521-535, 1993.
dc.identifier.fileWOSA1993MC77000001.pdf
dc.identifier.issn0100-8455
dc.identifier.urihttp://hdl.handle.net/11449/39250
dc.identifier.wosWOS:A1993MC77000001
dc.language.isoeng
dc.publisherSoc Brasil Genetica
dc.relation.ispartofRevista Brasileira de Genética
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCYTOMETRY AND KARYOMETRY IN TRYPANOSOMA-CRUZIen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjg/paboutj.htm
dcterms.rightsHolderSoc Brasil Genetica
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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