Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

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dc.contributor.authorPinto, Marcelo R.
dc.contributor.authorLucena, Malson N.
dc.contributor.authorFaleiros, Rogerio Oliveira
dc.contributor.authorAlmeida, Eduardo Alves [UNESP]
dc.contributor.authorMcNamara, John C.
dc.contributor.authorLeone, Francisco A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T15:28:35Z
dc.date.available2018-11-26T15:28:35Z
dc.date.issued2016-01-01
dc.description.abstractWe evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2 fold, respectively, after exposure to 2.0 mmol L-1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase alpha-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L-1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry. (C) 2015 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Campus Sao Jose Rio Preto, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Campus Sao Jose Rio Preto, Sao Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado do Amazonas
dc.description.sponsorshipIdCNPq: CNPq 560501/2101-2
dc.description.sponsorshipIdFAPESP: 2010/17534-0
dc.description.sponsorshipIdFAPESP: 2011/022537-0
dc.description.sponsorshipIdFAPESP: 2013/22605-1
dc.description.sponsorshipIdFundacao de Amparo a Pesquisa do Estado do Amazonas: INCT/CNPq/FAPEAM 573976/2008-2
dc.description.sponsorshipIdCNPq: 560501/2010-2
dc.description.sponsorshipIdCNPq: 302776/2011-7
dc.description.sponsorshipIdCNPq: 300564/2013-9
dc.description.sponsorshipIdCNPq: 307603/2014-8
dc.description.sponsorshipIdFAPESP: 2013/24252-9
dc.description.sponsorshipIdFAPESP: 2011/15776-9
dc.format.extent13-23
dc.identifierhttp://dx.doi.org/10.1016/j.aquatox.2015.10.021
dc.identifier.citationAquatic Toxicology. Amsterdam: Elsevier Science Bv, v. 170, p. 13-23, 2016.
dc.identifier.doi10.1016/j.aquatox.2015.10.021
dc.identifier.fileWOS000368564500002.pdf
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/11449/158678
dc.identifier.wosWOS:000368564500002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAquatic Toxicology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectAmmonia exposure
dc.subjectGill microsome
dc.subject(Na+, K+)-ATPase
dc.subjectV(H+)-ATPase
dc.subjectGene expression
dc.subjectOxidative enzymes
dc.subjectMacrobrachium amazonicum
dc.titleEffects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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