RECK-Mediated Inhibition of Glioma Migration and Invasion

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dc.contributor.authorSilveira Correa, Tatiana C.
dc.contributor.authorMassaro, Renato Ramos
dc.contributor.authorBrohem, Carla Abdo
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorLamers, Marcelo Lazzaron
dc.contributor.authorSantos, Marinilce Fagundes
dc.contributor.authorMaria-Engler, Silvya Stuchi
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:01:03Z
dc.date.available2014-05-20T14:01:03Z
dc.date.issued2010-05-01
dc.description.abstractRECK is an anti-tumoral gene whose activity has been associated with its inhibitory effects regulating MMP-2, MMP-9, and MT1-MMP. RECK level decreases as gliobastoma progresses, varying from less invasive grade II gliomas to very invasive human glioblastoma multiforme (GBM). Since RECK expression and glioma invasiveness show an inverse correlation, the aim of the present study is to investigate whether RECK expression would inhibit glioma invasive behavior. We conducted this study to explore forced RECK expression in the highly invasive T98G human GBM cell line. Expression levels as well as protein levels of RECK, MMP-2, MMP-9, and MT1-MMP were assessed by qPCR and immunoblotting in T98G/RECK+ cells. The invasion and migration capacity of RECK+ cells was inhibited in transwell and wound assays. Dramatic cytoskeleton modifications were observed in the T98G/RECK+ cells, when compared to control cells, such as the abundance of stress fibers (contractile actin-myosin II bundles) and alteration of lamellipodia. T98G/RECK+ cells also displayed phosphorylatecl focal adhesion kinase (P-FAK) in mature focal adhesions associated with stress fibers; whereas P-FAK in control cells was mostly associated with immature focal complexes. Interestingly, the RECK protein was predominantly localized at the leading edge of migrating cells, associated with membrane ruffles. Unexpectedly, introduced expression of RECK effectively inhibited the invasive process through rearrangement of actin filaments, promoting a decrease in migratory ability. This work has associated RECK tumor-suppressing activity with the inhibition of motility and invasion in this GBM model, which are two glioma characteristics responsible for the inefficiency of current available treatments. J. Cell. Biochem. 110: 52-61, 2010. (C) 2010 Wiley-Liss. Inc.en
dc.description.affiliationUniv São Paulo, Sch Pharmaceut Sci, Dept Clin Chem & Toxicol, São Paulo, Brazil
dc.description.affiliationIBILCE Univ State São Paulo, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, São Paulo, Brazil
dc.description.affiliationUnespIBILCE Univ State São Paulo, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 06/50915-1
dc.description.sponsorshipIdFAPESP: 05/51194-3
dc.description.sponsorshipIdFAPESP: 06/57508-2
dc.format.extent52-61
dc.identifierhttp://dx.doi.org/10.1002/jcb.22472
dc.identifier.citationJournal of Cellular Biochemistry. Hoboken: Wiley-liss, v. 110, n. 1, p. 52-61, 2010.
dc.identifier.doi10.1002/jcb.22472
dc.identifier.issn0730-2312
dc.identifier.lattes1445259468526188
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.urihttp://hdl.handle.net/11449/21572
dc.identifier.wosWOS:000277429700006
dc.language.isoeng
dc.publisherWiley-liss
dc.relation.ispartofJournal of Cellular Biochemistry
dc.relation.ispartofjcr2.959
dc.relation.ispartofsjr1,209
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectGLIOBLASTOMAen
dc.subjectRECK GENEen
dc.subjectCYTOSKELETONen
dc.subjectINVASIONen
dc.subjectMigrationen
dc.subjectMMPen
dc.titleRECK-Mediated Inhibition of Glioma Migration and Invasionen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-liss
unesp.author.lattes1445259468526188
unesp.author.orcid0000-0002-3257-3327[6]
unesp.author.orcid0000-0001-5296-5662[5]
unesp.author.orcid0000-0002-0970-4288[4]
unesp.author.orcid0000-0003-4771-6041[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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