Evaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of Toxoplasma gondii and Neospora caninum in maned wolves (Chrysocyon brachyurus)

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dc.contributor.authorSilva, D. A. O.
dc.contributor.authorVitaliano, S. N.
dc.contributor.authorMineo, T. W. P.
dc.contributor.authorFerreira, R. A.
dc.contributor.authorBevilacqua, E.
dc.contributor.authorMineo, J. R.
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionPrefeitura de Uberlândia
dc.date.accessioned2014-05-27T11:21:38Z
dc.date.available2014-05-27T11:21:38Z
dc.date.issued2005-10-01
dc.description.abstractUse of serological tests in the diagnosis of infectious diseases in wild animals has several limitations, primarily the difficulty of obtaining species-specific reagents. Wild canids, such as maned wolves (Chrysocyon brachyurus), are highly predisposed to infection by Toxoplasma gondii and, to a lesser extent, to Neospora caninum. The aim of the present study was to evaluate homologous, heterologous, and affinity conjugates in enzyme-linked immunosorbent assays (ELISAs) and indirect fluorescent antibody tests (IFATs) for detecting immunoglobulin (Ig) G antibodies against T. gondii and N. caninum in maned wolves. Serum samples were obtained from 59 captive animals in Brazil and tested by ELISA for T. gondii serology and IFAT for N. caninum serology using 3 different enzymatic and fluorescent conjugates: homologous (guinea pig anti-maned wolf IgG-peroxidase and -fluorescein isothiocyanate [FITC]), heterologous (rabbit anti-dog IgG-peroxidase and -FITC), and affinity (protein A-peroxidase and -FITC). Seropositivity to T. gondii was comparable among the homologous (69.5%), heterologous (74.6%), and affinity (71.2%) enzymatic conjugates. A significant positive correlation was found between the antibody levels determined by the 3 enzymatic conjugates. The highest mean antibody levels (ELISA index = 4.5) were observed with the protein A-peroxidase conjugate. The same seropositivity to N. caninum (8.5%) was found with the homologous and heterologous fluorescent conjugates, but protein A-FITC was not able to detect or confirm any positive samples with homologous or heterologous conjugates. Our results demonstrate that homologous, heterologous, and affinity conjugates might be used in ELISA for serological assays of T. gondii in wild canids, whereas for N. caninum infection, only the homologous or heterologous fluorescent conjugates have been shown to be useful. © American Society of Parasitologists 2005.en
dc.description.affiliationLaboratory of Immunoparasitology Universidade Federal de Uberlândia Campus Umuarama, Av. Para 1720, CEP 38400-902, Uberlândia, MG
dc.description.affiliationLaboratory of Immunoparasitology Faculdade de Ciências Agrárias e Veterinárias Campus de Jaboticabal, CEP 18884-900, Jaboticabal, SP
dc.description.affiliationLaboratory of Parasitology Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte, MG
dc.description.affiliationPrefeitura de Uberlândia, CEP 38408-900, Uberlândia, MG
dc.format.extent1212-1216
dc.identifierhttp://dx.doi.org/10.1645/GE-527R.1
dc.identifier.citationJournal of Parasitology, v. 91, n. 5, p. 1212-1216, 2005.
dc.identifier.doi10.1645/GE-527R.1
dc.identifier.issn0022-3395
dc.identifier.lattes6899226322073487
dc.identifier.orcid0000-0002-6243-0968
dc.identifier.scopus2-s2.0-28444479096
dc.identifier.urihttp://hdl.handle.net/11449/68432
dc.language.isoeng
dc.relation.ispartofJournal of Parasitology
dc.relation.ispartofjcr1.395
dc.relation.ispartofsjr0,662
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectfluorescein isothiocyanate
dc.subjectimmunoglobulin G
dc.subjectimmunoglobulin G antibody
dc.subjectperoxidase
dc.subjectprotein A
dc.subjectassessment method
dc.subjectcanid
dc.subjectinfectious disease
dc.subjectanimal disease
dc.subjectanimal experiment
dc.subjectantibody detection
dc.subjectblood sampling
dc.subjectBrazil
dc.subjectcorrelation analysis
dc.subjectenzyme linked immunosorbent assay
dc.subjectevaluation
dc.subjectfluorescence
dc.subjectfluorescent antibody technique
dc.subjectNeospora caninum
dc.subjectnonhuman
dc.subjectserodiagnosis
dc.subjectserum
dc.subjectToxoplasma gondii
dc.subjectwolf
dc.subjectAffinity Labels
dc.subjectAnimals
dc.subjectAnimals, Zoo
dc.subjectAntibodies, Protozoan
dc.subjectCanidae
dc.subjectCoccidiosis
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectImmunoglobulin G
dc.subjectNeospora
dc.subjectToxoplasma
dc.subjectToxoplasmosis, Animal
dc.subjectAnimalia
dc.subjectCanis familiaris
dc.subjectCavia porcellus
dc.subjectChrysocyon brachyurus
dc.subjectOryctolagus cuniculus
dc.titleEvaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of Toxoplasma gondii and Neospora caninum in maned wolves (Chrysocyon brachyurus)en
dc.typeArtigo
dcterms.licensehttp://www.bioone.org/page/terms_of_use
unesp.author.lattes6899226322073487[4]
unesp.author.orcid0000-0002-6243-0968[4]

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