SERS-based immunoassay for monitoring cortisol-related disorders

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dc.contributor.authorVilla, Javier E.L. [UNESP]
dc.contributor.authorGarcia, Isabel
dc.contributor.authorJimenez de Aberasturi, Dorleta
dc.contributor.authorPavlov, Valeri
dc.contributor.authorSotomayor, Maria D.P.T. [UNESP]
dc.contributor.authorLiz-Marzán, Luis M.
dc.contributor.institutionBasque Research and Technology Alliance (BRTA)
dc.contributor.institutionBiomateriales y Nanomedicina (CIBER-BBN)
dc.contributor.institutionBasque Foundation for Science
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T01:31:12Z
dc.date.available2020-12-12T01:31:12Z
dc.date.issued2020-10-01
dc.description.abstractAs a natural response to a stressful situation, the human body produces cortisol. For this reason, cortisol is also called “the stress hormone” and is considered to be the principal stress biomarker. Although cortisol response to stress is essential for survival, abnormal levels in biological fluids may represent serious health risks. In this work, we present a cortisol biosensor which relies on a highly sensitive technique (surface-enhanced Raman spectroscopy, SERS) and a specific recognition (immunoassay). Gold nanostars were used as SERS nanotags, since they provided a better response than nanorods or nanospheres. Using the same concept, two different immunoassay modalities were evaluated, using either magnetic beads or gold-coated glass slides decorated with cortisol antibodies as the capture substrates. The magnetically-assisted SERS immunoassay presented a better performance and was therefore selected to quantify cortisol content in biological fluids (urine and serum). Significant advantages of this assay were found over standard methods such as Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Enzyme-Linked Immunosorbent Assay (ELISA), including higher sensitivity and repeatability, minimum sample preparation, simplicity, and portability. Therefore, the proposed SERS immunoassay might be implemented as a highly efficient tool for in situ monitoring of human stress levels and cortisol-related disorders (e.g. Cushing's syndrome and Addison's disease).en
dc.description.affiliationCIC biomaGUNE Basque Research and Technology Alliance (BRTA), Paseo de Miramón 182
dc.description.affiliationCentro de Investigación Biomédica en Red Bioingeniería Biomateriales y Nanomedicina (CIBER-BBN), Paseo de Miramón 182
dc.description.affiliationIkerbasque Basque Foundation for Science
dc.description.affiliationInstituto de Química and INCT-DATREM Universidade Estadual Paulista (UNESP), Araraquara
dc.description.affiliationUnespInstituto de Química and INCT-DATREM Universidade Estadual Paulista (UNESP), Araraquara
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipEuropean Research Council
dc.description.sponsorshipAgencia Estatal de Investigación
dc.description.sponsorshipIdFAPESP: 2014/50945-4
dc.description.sponsorshipIdFAPESP: 2018/24202-5
dc.description.sponsorshipIdEuropean Research Council: 787510
dc.description.sponsorshipIdAgencia Estatal de Investigación: BIO2017-88030-R
dc.description.sponsorshipIdEuropean Research Council: ERC-AdG 4D-bioSERS
dc.description.sponsorshipIdAgencia Estatal de Investigación: MAT2017-86659-R
dc.description.sponsorshipIdAgencia Estatal de Investigación: MDM-2017-0720
dc.identifierhttp://dx.doi.org/10.1016/j.bios.2020.112418
dc.identifier.citationBiosensors and Bioelectronics, v. 165.
dc.identifier.doi10.1016/j.bios.2020.112418
dc.identifier.issn1873-4235
dc.identifier.issn0956-5663
dc.identifier.scopus2-s2.0-85088114338
dc.identifier.urihttp://hdl.handle.net/11449/199118
dc.language.isoeng
dc.relation.ispartofBiosensors and Bioelectronics
dc.sourceScopus
dc.subjectELISA
dc.subjectHydrocortisone
dc.subjectMagnetic separation
dc.subjectPlasmonics
dc.subjectSurface-enhanced Raman spectroscopy
dc.subjectUPLC-MS
dc.titleSERS-based immunoassay for monitoring cortisol-related disordersen
dc.typeArtigo

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