Crystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venom

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dc.contributor.authorSalvador, G. H. M. [UNESP]
dc.contributor.authorFernandes, C. A. H. [UNESP]
dc.contributor.authorCorrea, L. C. [UNESP]
dc.contributor.authorSantos-Filho, N. A.
dc.contributor.authorSoares, A. M.
dc.contributor.authorFontes, M. R. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:49:38Z
dc.date.available2014-05-20T13:49:38Z
dc.date.issued2009-10-01
dc.description.abstractCrotoxin B is a basic phospholipase A(2) found in the venom of several Crotalus durissus ssp. rattlesnakes and is one of the subunits that constitute crotoxin, the main component of the venom of these snakes. This heterodimeric toxin is related to important envenomation effects such as neurological disorders, myotoxicity and renal failure. Although crotoxin was first crystallized in 1938, the first structural data only became available in 2007 (for crotoxin B from C. durissus terrificus) and showed an ambiguous result for the biological assembly, which could be either dimeric or tetrameric. In this work, the crystallization, X-ray diffraction data collection at 2.2 angstrom resolution and molecular-replacement solution of a dimeric complex formed by two crotoxin B isoforms from C. durissus collilineatus venom is presented.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUSP, FCFRP, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipLaboratório Nacional de Luz Síncrotron (LNLS)
dc.format.extent1011-1013
dc.identifierhttp://dx.doi.org/10.1107/S1744309109032631
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell Publishing, Inc, v. 65, p. 1011-1013, 2009.
dc.identifier.doi10.1107/S1744309109032631
dc.identifier.fileWOS000270387700012.pdf
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/17699
dc.identifier.wosWOS:000270387700012
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venomen
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.author.orcid0000-0002-0344-6900[4]
unesp.author.orcid0000-0002-4634-6221[6]
unesp.author.orcid0000-0001-6515-6872[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Física e Biofísica - IBB