Optimization of xylanase biosynthesis by Aspergillus japonicus isolated from a Caatinga area in the Brazilian state of Bahia

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dc.contributor.authorSimões, Maria Lúcia Garcia [UNESP]
dc.contributor.authorTauk-Tornisielo, Samia Maria [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual do Sudoeste da Bahia (UESB)
dc.date.accessioned2014-05-27T11:21:53Z
dc.date.available2014-05-27T11:21:53Z
dc.date.issued2006-06-02
dc.description.abstractThe objective of this research was to investigate the potential of xylanase production by Aspergillus japonicus and to determine the effects of cultivation conditions in the process, aiming toward optimization of enzyme production. The best temperature, as well as the best carbon source, for biomass production was determined through an automated turbidimetric method (Bioscreen-C). The enzyme activity of this fungus was separately evaluated in two solid substrates (wheat and soybean bran) and in Vogel medium, adding other carbon sources. Temperature effects, cultivation time, and spore concentrations were also tested. The best temperature for enzyme and biomass production was 25°C; however, the best carbon source for growth (determined by the Bioscreen C) did not turn out to be a good inducer of xylanase production. Maximum xylanase activity was achieved when the fungus was cultivated in wheat bran (without the addition of any other carbon source) using a spore concentration of 1 × 107 spores/mL (25°C, pH 5.0, 120 h). A. japonicus is a good xylanase producer under the conditions presented in these assays. © 2006 Academic Journals.en
dc.description.affiliationCentro de Estudos Ambientais Universidade Estadual Paulista, Avenida 24-A, Rio Claro, SP 13506-900
dc.description.affiliationUniversidade Estadual do Sudoeste da Bahia, Km 4, Vitória da Conquista, Bahia, CEP 45050-020
dc.description.affiliationUnespCentro de Estudos Ambientais Universidade Estadual Paulista, Avenida 24-A, Rio Claro, SP 13506-900
dc.format.extent1135-1141
dc.identifierhttp://dx.doi.org/10.5897/AJB06.151
dc.identifier.citationAfrican Journal of Biotechnology, v. 5, n. 11, p. 1135-1141, 2006.
dc.identifier.doi10.5897/AJB06.151
dc.identifier.file2-s2.0-33745041560.pdf
dc.identifier.issn1684-5315
dc.identifier.scopus2-s2.0-33745041560
dc.identifier.urihttp://hdl.handle.net/11449/68912
dc.identifier.wosWOS:000238090400022
dc.language.isoeng
dc.relation.ispartofAfrican Journal of Biotechnology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAspergillus japonicusen
dc.subjectEnzyme optimizationen
dc.subjectXylanase activityen
dc.subjectcarbonen
dc.subjectxylan endo 1,3 beta xylosidaseen
dc.subjectAspergillusen
dc.subjectaspergillus japonicusen
dc.subjectbiomass productionen
dc.subjectbiosynthesisen
dc.subjectbranen
dc.subjectBrazilen
dc.subjectcarbon sourceen
dc.subjectconcentration (parameters)en
dc.subjectenzyme activityen
dc.subjectenzyme synthesisen
dc.subjectfungus isolationen
dc.subjectnonhumanen
dc.subjectsoybeanen
dc.subjectspecies cultivationen
dc.subjecttemperatureen
dc.subjectturbidimetryen
dc.subjectwheaten
dc.subjectFungien
dc.subjectGlycine maxen
dc.subjectTriticum aestivumen
dc.titleOptimization of xylanase biosynthesis by Aspergillus japonicus isolated from a Caatinga area in the Brazilian state of Bahiaen
dc.typeArtigo
dcterms.licensehttp://www.ajol.info/index.php/ajol/pages/view/OA

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