Comparative effects of acute-methionine loading on the plasma sulfur-amino acids in NAC-supplemented HIV+ patients and healthy controls

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dc.contributor.authorBurini, Roberto Carlos [UNESP]
dc.contributor.authorBorges-Santos, Maria Doroteia [UNESP]
dc.contributor.authorMoreto, Fernando [UNESP]
dc.contributor.authorYu, Yong- Ming
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionShriners Burns Hospital
dc.date.accessioned2018-12-11T17:19:15Z
dc.date.available2018-12-11T17:19:15Z
dc.date.issued2018-05-01
dc.description.abstractIn this study, an acute overloading of methionine (MetLo) was used to investigate the trassulfuration pathway response comparing healthy controls and HIV+ patients under their usual diet and dietary N-acetyl-l-cysteine (NAC) supplementation. MetLo (0.1 g Met/kg mass weight) was given after overnight fasting to 20 non-HIV+ control subjects (Co) and 12 HIV+ HAART-treated patients. Blood samples were taken before and after the MetLo in two different 7-day dietary situations, with NAC (1 g/day) or with their usual diet (UD). The amino acids (Met, Hcy, Cys, Tau, Ser, Glu and Gln) and GSH were determined by HPLC and their inflow rate into circulation (plasma) was estimated by the area under the curve (AUC). Under UD, the HIV+ had lower plasma GSH and amino acids (excepting Hcy) and higher oxidative stress (GSSG/GSH ratio), similar remethylation (RM: Me/Hcy + Ser ratio), transmethylation (TM; Hcy/Met ratio) and glutaminogenesis (Glu/Gln ratio), lower transsulfuration (TS: Cys/Hcy + Ser ratio) and Cys/Met ratio and, higher synthetic rates of glutathione (GG: GSH/Cys ratio) and Tau (TG: Tau/Cys ratio). NAC supplementation changed the HIV pattern by increasing RM above control, normalizing plasma Met and TS and, increasing plasma GSH and GG above controls. However, plasma Cys was kept always below controls probably, associatively to its higher consumption in GG (more GSSG than GSH) and TG. The failure of restoring normal Cys by MetLo, in addition to NAC, in HIV+ patients seems to be related to increased flux of Cys into GSH and Tau pathways, probably strengthening the cell-antioxidant capacity against the HIV progression (registered at http://www.clinicaltrials.gov, NCT00910442).en
dc.description.affiliationDepartment of Public Health Center for Nutritional and Physical Exercise Metabolism School of Medicine UNESP-Sao Paulo State University, Campus Botucatu
dc.description.affiliationDepartment of Surgery Harvard Medical School Massachusetts General Hospital Shriners Burns Hospital
dc.description.affiliationUnespDepartment of Public Health Center for Nutritional and Physical Exercise Metabolism School of Medicine UNESP-Sao Paulo State University, Campus Botucatu
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 99/01716-0
dc.format.extent569-576
dc.identifierhttp://dx.doi.org/10.1007/s00726-018-2538-2
dc.identifier.citationAmino Acids, v. 50, n. 5, p. 569-576, 2018.
dc.identifier.doi10.1007/s00726-018-2538-2
dc.identifier.file2-s2.0-85045033638.pdf
dc.identifier.issn1438-2199
dc.identifier.issn0939-4451
dc.identifier.scopus2-s2.0-85045033638
dc.identifier.urihttp://hdl.handle.net/11449/176148
dc.language.isoeng
dc.relation.ispartofAmino Acids
dc.relation.ispartofsjr1,135
dc.relation.ispartofsjr1,135
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCysteine metabolism
dc.subjectHIV+ patients
dc.subjectMethionine-load test
dc.subjectThiol redox system
dc.titleComparative effects of acute-methionine loading on the plasma sulfur-amino acids in NAC-supplemented HIV+ patients and healthy controlsen
dc.typeArtigo

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