Melatonin attenuates Her-2, p38 MAPK, p-AKT, and mTOR levels in ovarian carcinoma of ethanol-preferring rats

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dc.contributor.authorFerreira, Grazielle M. [UNESP]
dc.contributor.authorMartinez, Marcelo
dc.contributor.authorCamargo, Isabel Cristina Cherici [UNESP]
dc.contributor.authorDomeniconi, Raquel F. [UNESP]
dc.contributor.authorMartinez, Francisco Eduardo [UNESP]
dc.contributor.authorChuffa, Luiz Gustavo de Almeida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2015-08-21T17:53:08Z
dc.date.available2015-08-21T17:53:08Z
dc.date.issued2014
dc.description.abstractEpidermal growth factor receptors 2 (Her-2) and 4 (Her-4) are closely associated with ovarian cancer (OC) progression and metastasis, and a more complete understanding of these signaling pathways allow the development of new therapeutic strategies. Melatonin (Mel) is recognized as having several anticancer properties and has been reported to modulate Her-2 system in aggressive tumors. Here, we investigated OC and the role of Mel therapy on the Her-2- and Her-4-signaling pathway related to downstream molecules in an ethanol-preferring rat model. To induce OC, the left ovary was injected directly with a single dose of 100 µg 7,12-dimethylbenz(a)anthracene (DMBA) dissolved in 10 µL of sesame oil under the bursa. Right ovaries were used as sham-surgery controls. After developing OC, half of the animals received i.p. injections of Mel (200 µg/100 g b.w./day) for 60 days. While Mel therapy was unable to reduce Her-4 and phosphoinositide 3-kinase (PI3K) levels, it was able to suppress the OC-related increase in the levels of the Her-2, p38 mitogen-activated protein kinases (p38 MAPK), protein kinase B (phospho-AKT), and mammalian target of rapamycin (mTOR). In addition, Mel significantly attenuated the expression of Her-2, p38 MAPK, and p-AKT, which are involved in OC signaling during ethanol intake. Collectively, our results suggest that Mel attenuates the Her-2-signaling pathway in OC of ethanol-preferring rats, providing an effective contribution for further development of adjuvant therapies.en
dc.description.affiliationDepartment of Morphology and Pathology, UFSCar - Universidade Federal de São Carlos, São Carlos-SP, Brazil, 13565-905.
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Faculdade de Ciências e Letras de Assis, Assis, Av. Dom Antonio,2100 - Laboratório de Histologia e Embriologia, Jardim Universitário, CEP 19806900, SP, Brasil
dc.description.affiliationUnespDepartment of Anatomy, Biosciences Institute, UNESP - Univ. Estadual Paulista, Botucatu-SP, Brazil, 18618-970
dc.description.affiliationUnespDepartment of Biological Sciences, Faculty of Sciences and Letters, UNESP - Univ. Estadual Paulista, Assis-SP, Brazil, 19806-900.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/10309-9
dc.description.sponsorshipIdFAPESP: 2013/02466-7
dc.format.extent728-735
dc.identifierhttp://www.jcancer.org/v05p0728.htm
dc.identifier.citationJ Cancer, v. 5, n. 9, p. 728-735, 2014.
dc.identifier.doi10.7150/jca.10196
dc.identifier.fileISSN1837-9664-2014-05-09-728-735.pdf
dc.identifier.issn1837-9664
dc.identifier.lattes9804707674172774
dc.identifier.lattes1739564105219382
dc.identifier.lattes5121319676503034
dc.identifier.lattes3896494070099383
dc.identifier.lattes5481756528299469
dc.identifier.orcid0000-0003-2938-010X
dc.identifier.urihttp://hdl.handle.net/11449/126772
dc.language.isoeng
dc.relation.ispartofJ Cancer
dc.relation.ispartofjcr3.249
dc.relation.ispartofsjr1,159
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectOvarian canceren
dc.subjectMelatoninen
dc.subjectHer-2en
dc.subjectp38 MAPKen
dc.subjectp-AKTen
dc.subjectmTORen
dc.titleMelatonin attenuates Her-2, p38 MAPK, p-AKT, and mTOR levels in ovarian carcinoma of ethanol-preferring ratsen
dc.typeArtigo
unesp.author.lattes9804707674172774
unesp.author.lattes1739564105219382
unesp.author.lattes5121319676503034
unesp.author.lattes3896494070099383
unesp.author.lattes5481756528299469[4]
unesp.author.orcid0000-0003-2938-010X[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
unesp.departmentCiências Biológicaspt

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Artigos - Ciências Biológicas - FCLAS
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