Effect of thyroid hormone T3 on Myosin-Va expression in the central nervous system

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dc.contributor.authorde Souza Martins, Sheila Cristina
dc.contributor.authorRomao, Luciana Ferreira
dc.contributor.authorFaria, Jane Cristina
dc.contributor.authorde Holanda Afonso, Rosenilde Carvalho
dc.contributor.authorMurray, Samantha Angel
dc.contributor.authorPellizzon, Claudia Helena [UNESP]
dc.contributor.authorMercer, John A.
dc.contributor.authorCameron, Luiz-Claudio
dc.contributor.authorMoura-Neto, Vivaldo
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniv Fed Estado Rio de Janeiro
dc.contributor.institutionUniv Portsmouth
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMcLaughlin Res Inst
dc.contributor.institutionUniv Castelo Branco
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.date.accessioned2014-05-20T13:52:18Z
dc.date.available2014-05-20T13:52:18Z
dc.date.issued2009-06-12
dc.description.abstractThyroid hormones (THs) are essential for brain development, where they regulate gliogenesis, myelination, cell proliferation and protein synthesis. Hypothyroidism severely affects neuronal growth and establishment of synaptic connections. Triiodothyronine (T3), the biologically active form of TH, has a central function in these activities. So, Myosin-Va (Myo-Va), a molecular motor protein involved in vesicle and RNA transport, is a good candidate as a target for T3 regulation. Here, we analyzed Myo-Va expression in euthyroid and hypothyroid adult rat brains and synaptosomes. We observed a reduction of Myo-Va expression in cultured neural cells from newborn hypothyroid rat brain, while immunocytochemical experiments showed a punctate distribution of this protein in the cytoplasm of cells. Particularly, Myo-Va co-localized with microtubules in neurites, especially in their varicosities. Myo-Va immunostaining was stronger in astrocytes and neurons of controls when compared with hypothyroid brains. In addition, supplementation of astrocyte cultures with T3 led to increased expression of Myo-Va in cells from both euthyroid and hypothyroid animals, suggesting that T3 modulates Myo-Va expression in neural cells both in vivo and in vitro. We have further analyzed Myo-Va expression in U373 cells, a human glioblastoma line, and found the same punctate cytoplasmic protein localization. As in normal neural cells, this expression was also increased by T3, suggesting that the modulatory mechanism exerted by T3 over Myo-Va remains active on astrocyte tumor cells. These data, coupled with the observation that Myo-Va is severely affected in hypothyroidism, support the hypothesis that T3 activity regulates neural motor protein expression, taking Myo-Va as a model. As a consequence, reduced T3 activity could supposedly affect axonal transport and synaptic function, and could therefore explain disturbances seen in the hypothyroid brain. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Ciencias Biomed, Programa Anat, BR-21941590 Rio de Janeiro, Brazil
dc.description.affiliationUniv Fed Estado Rio de Janeiro, Lab Bioquim & Prot, BR-22290240 Rio de Janeiro, Brazil
dc.description.affiliationUniv Portsmouth, Sch Pharm & Biomed Sci, Portsmouth PO1 2DT, Hants, England
dc.description.affiliationUNESP, Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.affiliationMcLaughlin Res Inst, Great Falls, MT 59405 USA
dc.description.affiliationUniv Castelo Branco, Programa Posgrad Ciência Motricidade Humana, Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de Uberlândia (UFU), Inst Genet & Bioquim, Uberlandia, MG, Brazil
dc.description.affiliationUnespUNESP, Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipPrograma de Apoio aos Núcleos de Excelência (PRONEX)
dc.description.sponsorshipFUJB-UFRJ
dc.description.sponsorshipNIH
dc.description.sponsorshipIdCAPES: 542/2006
dc.description.sponsorshipIdPRONEX: 171.546/2006
dc.description.sponsorshipIdNIH: R01 GM066901
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2009.03.070
dc.identifier.citationBrain Research. Amsterdam: Elsevier B.V., v. 1275, p. 1-9, 2009.
dc.identifier.doi10.1016/j.brainres.2009.03.070
dc.identifier.issn0006-8993
dc.identifier.lattes0019393779801069
dc.identifier.orcid0000-0002-4494-4180
dc.identifier.urihttp://hdl.handle.net/11449/18691
dc.identifier.wosWOS:000267374900001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research
dc.relation.ispartofjcr3.125
dc.relation.ispartofsjr1,404
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectMyosin-Vaen
dc.subjectNeuronen
dc.subjectAstrocyteen
dc.subjectAstrogliomaen
dc.subjectHypothyroidismen
dc.subjectT3en
dc.titleEffect of thyroid hormone T3 on Myosin-Va expression in the central nervous systemen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0019393779801069[6]
unesp.author.orcid0000-0003-0307-4438[7]
unesp.author.orcid0000-0003-4180-2061[8]
unesp.author.orcid0000-0002-4494-4180[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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