Genetic diversity of Rhizoctonia solani AG-3 from potato and tobacco in North Carolina

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dc.contributor.authorCeresini, Paulo C. [UNESP]
dc.contributor.authorDavid Shew, H.
dc.contributor.authorVilgalys, Rytas J.
dc.contributor.authorCubeta, Marc A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNorth Carolina State University
dc.contributor.institutionDuke University
dc.date.accessioned2014-05-27T11:20:27Z
dc.date.available2014-05-27T11:20:27Z
dc.date.issued2002-05-01
dc.description.abstractAnastomosis group 3 (AG-3) of Rhizoctonia solani (teleomorph = Thanatephorus cucumeris) is frequently associated with diseases of potato (AG-3 PT) and tobacco (AG-3 TB). Although isolates of R. solani AG-3 from these two Solanaceous hosts are somatically related based on anastomosis reaction and taxonomically related based on fatty acid, isozyme and DNA characters, considerable differences are evident in their biology, ecology, and epidemiology. However, genetic diversity among field populations of R. solani AG-3 PT and TB has not been documented. In this study, the genetic diversity of field populations of R. solani AG-3 PT and AG-3 TB in North Carolina was examined using somatic compatibility and amplified fragment length polymorphism (AFLP) criteria. A sample of 32 isolates from potato and 36 isolates from tobacco were paired in all possible combinations on PDA plus activated charcoal and examined for their resulting somatic interactions. Twenty-eight and eight distinct somatic compatibility groups (SCG) were identified in the AG-3 PT and AG-3 TB samples, respectively. AFLP analyses indicated that each of the 32 AG-3 PT isolates had a distinct AFLP phenotype, whereas 28 AFLP phenotypes were found among the 36 isolates of AG-3 TB. None of the AG-3 PT isolates were somatically compatible or shared a common AFLP phenotype with any AG-3 TB isolate. Clones (i.e., cases where two or more isolates were somatically compatible and shared the same AFLP phenotype) were identified only in the AG-3 TB population. Four clones from tobacco represented 22% of the total population. All eight SCG from tobacco were associated with more than one AFLP phenotype. Compatible somatic interactions between AG-3 PT isolates occurred only between certain isolates from the same field (two isolates in each of four different fields), and when this occurred AFLP phenotypes were similar but not identical.en
dc.description.affiliationDepartamento de Biologia Faculdade de Engenharia Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), 15385-000, Ilha Solteira, Sao Paulo
dc.description.affiliationDepartment of Plant Pathology North Carolina State University, Raleigh, NC 27695
dc.description.affiliationDepartment of Biology Duke University, Durham, NC 27708
dc.description.affiliationUnespDepartamento de Biologia Faculdade de Engenharia Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), 15385-000, Ilha Solteira, Sao Paulo
dc.format.extent437-449
dc.identifierhttp://www.mycologia.org/content/94/3/437.short
dc.identifierhttp://dx.doi.org/10.2307/3761778
dc.identifier.citationMycologia, v. 94, n. 3, p. 437-449, 2002.
dc.identifier.doi10.2307/3761778
dc.identifier.file2-s2.0-0036587903.pdf
dc.identifier.issn0027-5514
dc.identifier.scopus2-s2.0-0036587903
dc.identifier.urihttp://hdl.handle.net/11449/66884
dc.identifier.wosWOS:000175866200009
dc.language.isoeng
dc.relation.ispartofMycologia
dc.relation.ispartofjcr2.762
dc.relation.ispartofsjr1,317
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectClonality
dc.subjectGenetic structure
dc.subjectMating
dc.subjectPopulation structure
dc.subjectRecombination
dc.subjectdisease
dc.subjectgenetic structure
dc.subjectgenetic variation
dc.subjectpopulation structure
dc.subjectHyphomycetes
dc.subjectMyxogastria
dc.subjectNicotiana tabacum
dc.subjectRhizoctonia
dc.subjectSolanum tuberosum
dc.subjectThanatephorus cucumeris
dc.titleGenetic diversity of Rhizoctonia solani AG-3 from potato and tobacco in North Carolinaen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Engenharia, Ilha Solteirapt

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Artigos - Biologia e Zootecnia - FEIS