Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes

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dc.contributor.authorPolettini, Jossimara [UNESP]
dc.contributor.authorVieira, Eliane Passarelli [UNESP]
dc.contributor.authorSantos, Mariana Perlati dos [UNESP]
dc.contributor.authorPeraçoli, José Carlos [UNESP]
dc.contributor.authorWitkin, Steven Sol [UNESP]
dc.contributor.authorSilva, Marcia da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCornell Univ
dc.date.accessioned2014-05-20T13:35:15Z
dc.date.available2014-05-20T13:35:15Z
dc.date.issued2012-04-01
dc.description.abstractObjective: To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis.Study design: A case control study that included 42 pregnant women not in labor in the following groups: PPROM (n = 28) and controls with intact membranes submitted to selective cesarean section at term (n = 14). Expression of IL-18 mRNA in chorioamniotic membranes was determined by real-time polymerase chain reaction, and IL-18 protein expression was measured by western blot. Histopathological analyses and immunolocalization of IL-18 by immunohistochemistry were also performed. Analyses were performed using the Mann-Whitney or Fisher's exact tests and the group effect was considered significant if the adjusted p-values were <0.05 and the magnitude of change was greater than 2-fold for mRNA expression.Results: IL-18 mRNA was present in 100% of samples and no difference in expression was observed between term vs. PPROM membranes (fold-change 0.12; p = 0.88). In the PPROM group, no difference was observed in IL-18 mRNA regarding gestational age (fold-change 0.11; p = 0.42) or the presence of histological chorioamnionitis (fold-change 0.26; p = 0.15). ProIL-18 was present in all samples. IL-18 was immunolocalized to amnion, chorion and decidua cells, with intense immunohistochemical staining at the choriodecidual junction.Conclusion: Chorioamniotic membranes are sources of IL-18 mRNA and proIL-18, and their expression is unrelated to PPROM or histological chorioamnionitis.en
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationCornell Univ, Dept Obstet & Gynecol, Weill Med Coll, New York, NY 10021 USA
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/55417-5
dc.description.sponsorshipIdFAPESP: 08/55517-0
dc.format.extent134-139
dc.identifierhttp://dx.doi.org/10.1016/j.ejogrb.2011.12.026
dc.identifier.citationEuropean Journal of Obstetrics & Gynecology and Reproductive Biology. Amsterdam: Elsevier B.V., v. 161, n. 2, p. 134-139, 2012.
dc.identifier.doi10.1016/j.ejogrb.2011.12.026
dc.identifier.fileWOS000302765400004.pdf
dc.identifier.issn0301-2115
dc.identifier.lattes8499437381595614
dc.identifier.urihttp://hdl.handle.net/11449/12112
dc.identifier.wosWOS:000302765400004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEuropean Journal of Obstetrics & Gynecology and Reproductive Biology
dc.relation.ispartofjcr1.809
dc.relation.ispartofsjr0,828
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectPreterm prelabor rupture of membranesen
dc.subjectInterleukin-18en
dc.subjectproIL-18en
dc.subjectHistological chorioamnionitisen
dc.titleInterleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8499437381595614
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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