The effect of temperature on the initial development of Brycon amazonicus Spix & Agassiz, 1829 as tool for micromanipulation of embryos

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dc.contributor.authorCristina da Silva, Regiane
dc.contributor.authorPereira dos Santos, Matheus
dc.contributor.authorSenhorini, José Augusto
dc.contributor.authorPaes, Maria Do Carmo Faria
dc.contributor.authorValentin, Fernanda Nogueira
dc.contributor.authorFujimoto, Takafumi
dc.contributor.authordo Nascimento, Nivaldo Ferreira
dc.contributor.authorYasui, George Shigueki
dc.contributor.authorNakaghi, Laura Satiko Okada
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:23:38Z
dc.date.available2018-12-11T17:23:38Z
dc.date.issued2017-09-20
dc.description.abstractPrimordial germ cell (PGC) transplant is a promising tool in aquaculture; however, successful use of this technique requires in depth knowledge of the early stages of embryo and larval development. The aim of this study was to analyse the effect of different temperatures (22, 26, and 30°C) on the early development of B. amazonicus. The newly fertilized eggs were distributed into tanks with controlled temperature and oxygenation. Samples were collected at pre-established times and analysed under light and fluorescence microscopy. Temperature influenced the speed and duration of each stage of early development, including hatching time. The highest pronuclei fusion rate was observed 8 min post-fertilization (mpf) at 22 and 26°C, and 6 mpf at 30°C. The duration of the 512–1000 blastomeres phase during in the blastocyst stage was 1 h 30 min at 22°C, and 25 min at 26 and 30°C. Hatching occurred at 24 h 30 mpf at 22°C, 16 h post-fertilization (hpf) at 26°C, and 11 h 30 mpf at 30°C. The rate of morphologically normal larvae was 88.34% at 22°C, 90.49% at 26°C, and 73% at 30°C. Malformations of the head, yolk sac, heart, and tail were observed in all temperatures. Nevertheless, B. amazonicus embryos were able to develop satisfactory in all three temperatures tested. These results enable embryo manipulation at different temperatures to optimize the micromanipulation time of embryos and larvae for biotechnological studies.en
dc.description.affiliationUNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP Brazil.
dc.description.affiliationUNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
dc.description.affiliationFish Biotechnology Laboratory, National Centre for Research and Continental Fish Conservation, Chico Mendes Institute of Biodiversity Conservation (CEPTA/ICMBio), Rodovia Pref. Euberto Nemesio Pereira de Godoy, Km 6,5, CEP 13630-970. Pirassununga-SP, Brazil.
dc.description.affiliationLaboratory Animal Histology and Embryology, Department of Animal Morphology and Physiology, UNESP- São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900 Jaboticabal, SP, Brazil.
dc.description.affiliationFaculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, 041-8611 Hakodate, Japan.
dc.format.extent1-15
dc.identifierhttp://dx.doi.org/10.1017/S096719941700051X
dc.identifier.citationZygote, p. 1-15.
dc.identifier.doi10.1017/S096719941700051X
dc.identifier.file2-s2.0-85030836997.pdf
dc.identifier.issn1469-8730
dc.identifier.issn0967-1994
dc.identifier.lattes9203413733944127
dc.identifier.lattes9203413733944127
dc.identifier.scopus2-s2.0-85030836997
dc.identifier.urihttp://hdl.handle.net/11449/177048
dc.language.isoeng
dc.relation.ispartofZygote
dc.relation.ispartofsjr0,387
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBlastula
dc.subjectCell transplantation
dc.subjectChimera
dc.subjectEmbryo
dc.subjectGerm cell
dc.titleThe effect of temperature on the initial development of Brycon amazonicus Spix & Agassiz, 1829 as tool for micromanipulation of embryosen
dc.typeArtigo
unesp.author.lattes9203413733944127

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Jaboticabal - CAUNESP - Centro de Aquicultura da Unesp
Artigos - Morfologia e Fisiologia Animal - FCAV