Venomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom proteins and splicing variants

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dc.contributor.authorViala, Vincent Louis
dc.contributor.authorHildebrand, Diana
dc.contributor.authorTrusch, Maria
dc.contributor.authorFucase, Tamara Mieco
dc.contributor.authorSciani, Juliana Mozer
dc.contributor.authorPimenta, Daniel Carvalho
dc.contributor.authorArni, Raghuvir Krishnaswamy [UNESP]
dc.contributor.authorSchlüter, Hartmut
dc.contributor.authorBetzel, Christian
dc.contributor.authorMirtschin, Peter
dc.contributor.authorDunstan, Nathan
dc.contributor.authorSpencer, Patrick Jack
dc.contributor.institutionInstituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.contributor.institutionUniversitätsklinikum Hamburg-Eppendorf
dc.contributor.institutionUniversität Hamburg
dc.contributor.institutionInstituto Butantan
dc.date.accessioned2015-12-07T15:33:26Z
dc.date.available2015-12-07T15:33:26Z
dc.date.issued2015
dc.description.abstractThe eastern brown snake is the predominant cause of snakebites in mainland Australia. Its venom induces defibrination coagulopathy, renal failure and microangiopathic hemolytic anemia. Cardiovascular collapse has been described as an early cause of death in patients, but, so far, the mechanisms involved have not been fully identified. In the present work, we analysed the venome of Pseudonaja textilis by combining high throughput proteomics and transcriptomics, aiming to further characterize the components of this venom. The combination of these techniques in the analysis and identification of toxins, venom proteins and putative toxins allowed the sequence description and the identification of the following: prothrombinase coagulation factors, neurotoxic textilotoxin phospholipase A2 (PLA2) subunits and acidic PLA2, three-finger toxins (3FTx) and the Kunitz-type protease inhibitor textilinin, venom metalloproteinase, C-type lectins, cysteine rich secretory proteins, calreticulin, dipeptidase 2, as well as evidences of Heloderma lizard peptides. Deep data-mining analysis revealed the secretion of a new transcript variant of venom coagulation factor 5a and the existence of a splicing variant of PLA2 modifying the UTR and signal peptide from a same mature protein. The transcriptome revealed the diversity of transcripts and mutations, and also indicates that splicing variants can be an important source of toxin variation.en
dc.description.affiliationInstituto de Pesquisas Energéticas e Nucleares, Centro de Biotecnologia
dc.description.affiliationUniversitätsklinikum Hamburg-Eppendorf, Institut für Klinische Chemie
dc.description.affiliationUniversität Hamburg, Institut für Biochemie und Molekularbiologie
dc.description.affiliationInstituto Butantan, Laboratório de Bioquímica e Biofísica
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenadação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2009/10305-8
dc.description.sponsorshipIdCAPES: 364/11
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2015.06.005
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology, 2015.
dc.identifier.doi10.1016/j.toxicon.2015.06.005
dc.identifier.issn1879-3150
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.pubmed26079951
dc.identifier.urihttp://hdl.handle.net/11449/131286
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinology
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectProteomeen
dc.subjectPseudonaja textilisen
dc.subjectSplicing varianten
dc.subjectToxinen
dc.subjectTranscriptomeen
dc.titleVenomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom proteins and splicing variantsen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes9162508978945887[7]
unesp.author.orcid0000-0003-2460-1145[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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