Possible interspecific origin of the B chromosome of Hypsiboas albopunctatus (Spix, 1824) (Anura, Hylidae), revealed by microdissection, chromosome painting, and reverse hybridisation

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dc.contributor.authorGruber, Simone Lilian [UNESP]
dc.contributor.authorDiniz, Débora
dc.contributor.authorSobrinho-Scudeler, Patrícia Elda [UNESP]
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.authorHaddad, Célio Fernando Baptista [UNESP]
dc.contributor.authorKasahara, Sanae [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUESB, Universidade Estadual do Sudoeste da Bahia, Departamento de Ciências Biológicas
dc.date.accessioned2018-12-11T16:56:28Z
dc.date.available2018-12-11T16:56:28Z
dc.date.issued2014-01-01
dc.description.abstractThe B chromosome in the hylid Hypsiboas albopunctatus (2n = 22 + B) is small, almost entirely composed of C-positive heterochromatin, and does not pair with any chromosome of the A complement. B probe, obtained by microdissection and DOP-PCR amplification, was used to search for homology between the B and regular chromosomes of H. albopunctatus and of the related species H. raniceps (Cope, 1862). Reverse hybridisation was also carried out in the investigation. The B probe exclusively painted the supernumerary, not hybridising any other chromosomes in H. albopunctatus, but all H. raniceps chromosomes showed small labelling signals. This result might be an indication that differences exist between the repetitive sequences of A and B chromosomes of H. albopunctatus, and that the chromosomes of H. raniceps and the heterochromatin of the B chromosome of H. albopunctatus are enriched with the same type of repetitive DNA. In meiotic preparations, the B labelled about 30% of scored spermatids, revealing a non-mendelian inheritance, and the painted B in micronucleus suggests that the supernumerary is eliminated from germ line cells. Although our results could suggest an interespecific origin of the B at first sight, further analysis on its repetitive sequences is still necessary. Nevertheless, the accumulation of repetitive sequences, detected in another species, even though closely related, remains an intriguing question. © Simone L. Gruber et al.en
dc.description.affiliationUNESP, Universidade Estadual Paulista, Instituto de Biociências, Departamento de Biologia, Av. 24A 1515, 13506-900, Rio Claro, SP
dc.description.affiliationUESB, Universidade Estadual do Sudoeste da Bahia, Departamento de Ciências Biológicas, Rua José Moreira Sobrinho s/n, Jequiezinho, 45206-190, Jequié, BA
dc.description.affiliationUNESP, Universidade Estadual Paulista, Instituto de Biociências, Departamento de Morfologia, Distrito de Rubião Júnior s/n, 18618-970, Botucatu, SP
dc.description.affiliationUNESP, Universidade Estadual Paulista, Instituto de Biociências, Departamento de Zoologia, Av. 24A 1515, 13506-900, Rio Claro, SP
dc.description.affiliationUnespUNESP, Universidade Estadual Paulista, Instituto de Biociências, Departamento de Biologia, Av. 24A 1515, 13506-900, Rio Claro, SP
dc.description.affiliationUnespUNESP, Universidade Estadual Paulista, Instituto de Biociências, Departamento de Morfologia, Distrito de Rubião Júnior s/n, 18618-970, Botucatu, SP
dc.description.affiliationUnespUNESP, Universidade Estadual Paulista, Instituto de Biociências, Departamento de Zoologia, Av. 24A 1515, 13506-900, Rio Claro, SP
dc.format.extent185-197
dc.identifierhttp://dx.doi.org/10.3897/CompCytogen.v8i3.7771
dc.identifier.citationComparative Cytogenetics, v. 8, n. 3, p. 185-197, 2014.
dc.identifier.doi10.3897/CompCytogen.v8i3.7771
dc.identifier.issn1993-078X
dc.identifier.issn1993-0771
dc.identifier.scopus2-s2.0-84906080442
dc.identifier.urihttp://hdl.handle.net/11449/171661
dc.language.isoeng
dc.relation.ispartofComparative Cytogenetics
dc.relation.ispartofsjr0,431
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectFISH
dc.subjectGISH
dc.subjectHeterochromatin
dc.subjectHylidae family
dc.subjectRepetitive DNA
dc.subjectReplication banding
dc.subjectSupernumerary chromosome
dc.titlePossible interspecific origin of the B chromosome of Hypsiboas albopunctatus (Spix, 1824) (Anura, Hylidae), revealed by microdissection, chromosome painting, and reverse hybridisationen
dc.typeArtigo
unesp.author.lattes0458077399058762[5]
unesp.author.orcid0000-0002-7044-5764[5]

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Artigos - Biologia - IBRC
Artigos - Morfologia - IBB
Artigos - Zoologia - IBRC