Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse

Página do item simplificado
dc.contributor.authorCredendino, Sara Carmela
dc.contributor.authorLewin, Nicole
dc.contributor.authorDe Oliveira, Miriane
dc.contributor.authorBasu, Swaraj
dc.contributor.authorD'Andrea, Barbara
dc.contributor.authorAmendola, Elena
dc.contributor.authorDi Guida, Luigi
dc.contributor.authorNardone, Antonio
dc.contributor.authorSanges, Remo
dc.contributor.authorDe Felice, Mario
dc.contributor.authorDe Vita, Gabriella
dc.contributor.institutionUniversità degli Studi di Napoli Federico II
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Gothenburg
dc.contributor.institutionStazione Zoologica Anton Dohrn
dc.date.accessioned2022-04-29T08:45:19Z
dc.date.available2022-04-29T08:45:19Z
dc.date.issued2017-01-01
dc.description.abstractlncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described. Klhl14-AS is expressed with a peculiar pattern, characterized by diverse relative abundance of its isoforms in different mouse tissues. We examine the whole expression level of Klhl14-AS in a panel of adult mouse tissues, showing that it is expressed in the thyroid, lung, kidney, testis, ovary, brain, and spleen, although at different levels. In situ hybridization analysis reveals that, in the context of each organ, Klhl14-AS shows a cell type-specific expression. Interestingly, databases report a similar expression profile for human Klhl14-AS. Our observations suggest that this lncRNA could play cell type-specific roles in several organs and pave the way for functional characterization of this gene in appropriate biological contexts.en
dc.description.affiliationDipartimento di Medicina Molecolare e Biotecnologie Mediche Università degli Studi di Napoli Federico II, Via S. Pansini 5
dc.description.affiliationDepartment of Internal Medicine Botucatu School of Medicine University of São Paulo State
dc.description.affiliationDepartment of Medical Biochemistry and Cell Biology Institute of Biomedicine Sahlgrenska Academy University of Gothenburg
dc.description.affiliationDipartimento di Sanità Pubblica Università degli Studi di Napoli Federico II, Via S. Pansini 5
dc.description.affiliationBiology and Evolution of Marine Organisms Stazione Zoologica Anton Dohrn, Villa Comunale 1
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipIdEuropean Commission: RNA-REGULOMICS 318981
dc.identifierhttp://dx.doi.org/10.1155/2017/9769171
dc.identifier.citationInternational Journal of Genomics, v. 2017.
dc.identifier.doi10.1155/2017/9769171
dc.identifier.issn2314-4378
dc.identifier.issn2314-436X
dc.identifier.scopus2-s2.0-85030632928
dc.identifier.urihttp://hdl.handle.net/11449/231409
dc.language.isoeng
dc.relation.ispartofInternational Journal of Genomics
dc.sourceScopus
dc.titleTissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouseen
dc.typeArtigo
unesp.author.orcid0000-0002-7302-1174[11]

Arquivos

Coleções

Artigos - Clínica Médica - FMB