Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats

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dc.contributor.authorde Souza Faloni, Ana Paula
dc.contributor.authorCerri, Estela Sasso [UNESP]
dc.contributor.authorGodoy Rocha, Fernanda Regina
dc.contributor.authorKatchburian, Eduardo
dc.contributor.authorCerri, Paulo Sérgio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:46:51Z
dc.date.available2014-05-20T13:46:51Z
dc.date.issued2012-01-01
dc.description.abstractThis study investigated structural and functional features of apoptotic alveolar bone osteoclasts in estrogen-treated rats. For this purpose, 15 female rats 22 days old were divided into three groups: Estrogen (EG), Sham (SG) and Control (CG). The rats of EG received daily intramuscular injection of estrogen for 7 days. The SG received only the oil vehicle. Maxillary fragments containing alveolar bone were removed and processed for light and transmission electron microscopy. Area (OcA) and number of nuclei (OcN) and bone resorption surface per TRAP-positive osteoclasts (BS/OC) were obtained. Vimentin, caspase-3 and MMP-9 immunoreactions, TUNEL/TRAP and MMP-9/TUNEL combined reactions were performed. In EG, the OcA, OcN and BS/Oc were reduced. Moreover, osteoclasts showed cytoplasm immunolabelled by caspase-3 and a different pattern of vimentin expression in comparison with CG and SG. MMP-9 expression was not affected by estrogen and the TUNEL-positive osteoclasts were MMP-9-immunolabelled. In EG, ultrastructural images showed that apoptotic osteoclasts did not exhibit ruffled borders or clear zones and were shedding mononucleated portions. TRAP-positive structures containing irregular and dense chromatin were partially surrounded by fibroblast-like cells. In conclusion, the reduction in the BS/Oc may be due to reduction in OcA and OcN; these effects seem to be related to vimentin disarrangement rather than to an interference of estrogen with osteoclast MMP-9 expression. Osteoclast apoptosis involves caspase-3 activity and vimentin degradation; these cells release portions containing one apoptotic nucleus and, subsequently, undergo fragmentation, giving rise to apoptotic bodies.en
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Morphol, Sch Dent, Lab Histol & Embryol, BR-14801903 São Paulo, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Morphol, Sch Dent, Lab Histol & Embryol, BR-14801903 São Paulo, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 04/09898-0
dc.format.extent77-85
dc.identifierhttp://dx.doi.org/10.1111/j.1469-7580.2011.01449.x
dc.identifier.citationJournal of Anatomy. Malden: Wiley-blackwell, v. 220, n. 1, p. 77-85, 2012.
dc.identifier.doi10.1111/j.1469-7580.2011.01449.x
dc.identifier.issn0021-8782
dc.identifier.lattes4455630076841302
dc.identifier.lattes3278495911207882
dc.identifier.orcid0000-0001-5756-5828
dc.identifier.urihttp://hdl.handle.net/11449/16609
dc.identifier.wosWOS:000297947100008
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Anatomy
dc.relation.ispartofjcr2.479
dc.relation.ispartofsjr1,078
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectapoptosisen
dc.subjectestradiolen
dc.subjectMMP-9en
dc.subjectosteoclasten
dc.subjectvimentinen
dc.titleStructural and functional changes in the alveolar bone osteoclasts of estrogen-treated ratsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes4455630076841302
unesp.author.lattes3278495911207882[5]
unesp.author.orcid0000-0001-7386-1280[1]
unesp.author.orcid0000-0001-5756-5828[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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