Evidence of Leishmania spp. antibodies and DNA in bush dogs (Speothos venaticus) in Brazil

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dc.contributor.authorFelix Lima, Valeria Marcal [UNESP]
dc.contributor.authorFattori, Karina Reinaldo [UNESP]
dc.contributor.authorMichelin, Aparecida de Fatima [UNESP]
dc.contributor.authorNogueira, Fabio dos Santos [UNESP]
dc.contributor.authorde Oliveira e Souza, Lucio
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCtr Conservacao Fauna Silvestre
dc.date.accessioned2014-05-20T15:31:15Z
dc.date.available2014-05-20T15:31:15Z
dc.date.issued2009-03-01
dc.description.abstractThe municipality of Ilha Solteira, São Paulo, Brazil is an endemic area of leishmaniasis. At the Companhia Energetica de São Paulo (CESP) Wild Animal Center of Ilha Solteira, two bush dogs (Speothos venaticus) showed clinical signs of this disease. The amastigote form of Leishmania was detected in lymph-node smears taken by fine-needle biopsy. In addition, serum samples from both animals, screened with an enzyme-linked immunosorbent assay (ELISA). were positive for anti-Leishmania antibodies. Moreover, tissue samples from one of the bush dogs were evaluated for the presence of Leishmania DNA by means of a polymerase chain reaction (PCR). DNA of the parasite was indeed detected in the tissue samples of the liver and the lymph nodes; however, no DNA from the parasite was detected in samples of the skin and spleen. These findings confirm a Leishmania infection in bush dogs (S. venaticus).en
dc.description.affiliationUniv Estadual Paulista, Dept Clin Cirurgia & Reprod Anim, Fac Odontol Med Vet, BR-16050400 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Curso Posgrad Microbiol Agropecuaria, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Curso Posgrad Med Vet, Fac Med Vet & Zootecn, BR-18618000 São Paulo, Brazil
dc.description.affiliationCtr Conservacao Fauna Silvestre, CESP, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Clin Cirurgia & Reprod Anim, Fac Odontol Med Vet, BR-16050400 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Curso Posgrad Microbiol Agropecuaria, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Curso Posgrad Med Vet, Fac Med Vet & Zootecn, BR-18618000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent91-94
dc.identifierhttp://dx.doi.org/10.1638/2008-0043.1
dc.identifier.citationJournal of Zoo and Wildlife Medicine. Yulee: Amer Assoc Zoo Veterinarians, v. 40, n. 1, p. 91-94, 2009.
dc.identifier.doi10.1638/2008-0043.1
dc.identifier.issn1042-7260
dc.identifier.urihttp://hdl.handle.net/11449/40440
dc.identifier.wosWOS:000264775800012
dc.language.isoeng
dc.publisherAmer Assoc Zoo Veterinarians
dc.relation.ispartofJournal of Zoo and Wildlife Medicine
dc.relation.ispartofjcr0.684
dc.relation.ispartofsjr0,424
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbush dogen
dc.subjectELISAen
dc.subjectLeishmania spp.en
dc.subjectPCRen
dc.subjectSpeothos venaticusen
dc.titleEvidence of Leishmania spp. antibodies and DNA in bush dogs (Speothos venaticus) in Brazilen
dc.typeArtigo
dcterms.rightsHolderAmer Assoc Zoo Veterinarians
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Biologia - FCAV