Serological and molecular techniques applied for identification of plasmodium spp. In blood samples from nonhuman primates

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dc.contributor.authorFigueiredo, Mayra Araguaia Pereira
dc.contributor.authorDi Santi, Silvia Maria
dc.contributor.authorManrique, Wilson Gómez
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.institutionUniversidade Federal de Rondônia – UNIR
dc.contributor.institutionSuperintendência de Controle de Endemias – SUCEN
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:00:21Z
dc.date.available2019-10-06T16:00:21Z
dc.date.issued2018-07-01
dc.description.abstractThe aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhão, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs were analyzed: 141 from captive animals at a Wildlife Screening Center (CETAS) and 20 from free-living animals in a private reserve. The techniques used were microscopy, rapid diagnostic test (RDT), Indirect fluorescent antibody test (IFAT) and molecular techniques (semi-nested PCR, quantitative real-time PCR and LAMP). Two serological methods (dot-ELISA and indirect ELISA) were also standardized with rhoptry protein-soluble antigen of P. falciparum and P. berghei. Trophozoite forms of Plasmodium sp. were identified on slides from five different animals. No samples were positive through RDT and LAMP. Four samples were seropositive for P. malariae through IFAT. The samples showed low reactivity to ELISA. Plasmodium sp. was detected in 34.16% (55/161) of the samples using qPCR based on the 18S rRNA gene. After sequencing, two samples showed 100% identityl to P. malariae, one showed 97% identity to Plasmodium sp. ZOOBH and one showed 99% identity to P. falciparum. PCR was shown to be the most sensitive technique for diagnosing Plasmodium in NHP samples.en
dc.description.affiliationLaboratório de Parasitologia Animal Curso de Medicina Veterinária Universidade Federal de Rondônia – UNIR
dc.description.affiliationCentro de Estudos da Malária Superintendência de Controle de Endemias – SUCEN
dc.description.affiliationDepartamento de Saúde do Estado de São Paulo Instituto de Medicina Tropical de São Paulo – IMTSP Universidade de São Paulo – USP
dc.description.affiliationLaboratório de Patologia Veterinária Curso de Medicina Veterinária Universidade Federal de Rondônia – UNIR
dc.description.affiliationLaboratório de Imunoparasitologia Faculdade de Ciências Agrárias e Veterinárias – FCAV Universidade Estadual Paulista – UNESP
dc.description.affiliationUnespLaboratório de Imunoparasitologia Faculdade de Ciências Agrárias e Veterinárias – FCAV Universidade Estadual Paulista – UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipInstituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis
dc.description.sponsorshipIdFAPESP: 10/12820-4
dc.description.sponsorshipIdFAPESP: 12/03961-9
dc.format.extent363-376
dc.identifierhttp://dx.doi.org/10.1590/s1984-296120180043
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 27, n. 3, p. 363-376, 2018.
dc.identifier.doi10.1590/s1984-296120180043
dc.identifier.fileS1984-29612018000300363.pdf
dc.identifier.issn0103-846X
dc.identifier.scieloS1984-29612018000300363
dc.identifier.scopus2-s2.0-85054775093
dc.identifier.urihttp://hdl.handle.net/11449/188196
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject18S rRNA
dc.subjectNew world monkeys
dc.subjectPlasmodium brasilianum
dc.subjectPlasmodium malariae
dc.subjectSimian malaria
dc.subjectZoonosis
dc.titleSerological and molecular techniques applied for identification of plasmodium spp. In blood samples from nonhuman primatesen
dc.titleTécnicas sorológicas e moleculares aplicadas na identificação de Plasmodium spp. em amostras de primatas não humanospt
dc.typeArtigo

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