Effects of Different Swimming Exercise Intensities on Bone Tissue Composition in Mice: A Raman Spectroscopy Study

Página do item simplificado
dc.contributor.authorda Silva, Fabiano Fernandes
dc.contributor.authorde Souza, Renato Aparecido
dc.contributor.authorTavares Pacheco, Marcos Tadeu
dc.contributor.authorRibeiro, Wellington
dc.contributor.authorSouza Rodrigues da Silva, Marcos Augusto
dc.contributor.authorMiranda, Humberto
dc.contributor.authorCastillo Salgado, Miguel Angel [UNESP]
dc.contributor.authorde Melo Castilho, Julio Cezar [UNESP]
dc.contributor.authorSilveira, Landulfo
dc.contributor.institutionUniv Camilo Castelo Branco UNICASTELO
dc.contributor.institutionInst Fed Educ Ciência & Tecnol Minas
dc.contributor.institutionUniv Vale Paraiba UNIVAP
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:04:43Z
dc.date.available2014-05-20T14:04:43Z
dc.date.issued2011-04-01
dc.description.abstractObjective: Raman spectroscopy was employed to evaluate the effect of different swimming intensities on femoral bone composition in an animal model. Background Data: Intense swimming exercise may affect bone mineralization, and Raman technique has been shown to be effective in evaluating tissue composition (phosphate minerals and carbonate apatites - bands at 960 and 1170 cm(-1), as well as collagen matrix - amide I band at 1660 cm(-1)). Materials and Methods: Eighteen female Swiss Webster mice were separated into three groups (n = 6 per group) of sedentary (SED), and swimming with an intensity of 40% (PT-40) and 80% (PT-80) of the maximum load, with 6 weeks of training. Near-infrared Raman spectra (830nm wavelength and 80mW laser power) were obtained with a dispersive Raman spectrometer using a CCD camera and imaging spectrograph with 30-s integration time. Spectra were collected in the medial and lateral diaphysis of the femur and principal components analysis (PCA) was employed to extract features of the Raman bands of bone and to perform quantitative analysis. Results: PC1 vector resembles Raman spectra and carries information about apatite minerals and some contribution from organic matrix. A statistically significant difference was found in the PC1 scores (ANOVA, p < 0.05), indicating lower mineral concentrations in the femur in both the PT-40 and PT-80 groups compared to the SED group. These results corroborated with the radiographic assessment of bone density. Conclusion: Raman technique associated with PCA statistics showed that intense swimming exercise may affect bone mineralization and remodeling in a mouse model of training.en
dc.description.affiliationUniv Camilo Castelo Branco UNICASTELO, Ctr Biomed Engn, BR-12247004 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationInst Fed Educ Ciência & Tecnol Minas, Grp Estudo & Pesquisa Ciência Saúde GEP CS, Muzambinho, MG, Brazil
dc.description.affiliationUniv Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Sch Phys Educ & Sports, BR-21941 Rio de Janeiro, RJ, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Fac Odontol Sao Jose Campos, Sao Jose Dos Campos, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Fac Odontol Sao Jose Campos, Sao Jose Dos Campos, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 305610/2008-2
dc.format.extent217-225
dc.identifierhttp://dx.doi.org/10.1089/pho.2010.2784
dc.identifier.citationPhotomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc., v. 29, n. 4, p. 217-225, 2011.
dc.identifier.doi10.1089/pho.2010.2784
dc.identifier.fileWOS000289074200002.pdf
dc.identifier.issn1549-5418
dc.identifier.lattes8235700830441386
dc.identifier.urihttp://hdl.handle.net/11449/22705
dc.identifier.wosWOS:000289074200002
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofPhotomedicine and Laser Surgery
dc.relation.ispartofjcr1.620
dc.relation.ispartofsjr0,443
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleEffects of Different Swimming Exercise Intensities on Bone Tissue Composition in Mice: A Raman Spectroscopy Studyen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/archpolicy/journal-of-womens-health/42/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.lattes8235700830441386
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000289074200002.pdf
Tamanho:
3.48 MB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biociências e Diagnóstico Bucal - ICT