Immunoassay quantification using surface-enhanced fluorescence (SEF) tags

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dc.contributor.authorCamacho, S. A. [UNESP]
dc.contributor.authorSobral-Filho, R. G.
dc.contributor.authorAoki, P. H.B. [UNESP]
dc.contributor.authorConstantino, C. J.L. [UNESP]
dc.contributor.authorBrolo, A. G.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Victoria
dc.date.accessioned2018-12-11T17:13:34Z
dc.date.available2018-12-11T17:13:34Z
dc.date.issued2017-08-07
dc.description.abstractFluorescence-based immonoassays are widely used in several areas, ranging from basic biomedical research to disease diagnostics. A variety of new probes have been developed recently to address some limitations in typical assays performed with organic dyes. Ideally, new fluorescence tags that allow quantification with a low limit of detection are highly desired. In this work, the surface-enhanced fluorescence (SEF) phenomenon was explored in the development of tags for Immunoglobulin-M (IgM) detection. Shell-isolated gold nanoparticles (Au-SHINs) with 100 nm core size and a 10 nm silica shell were synthesized. These particles contain an outermost thin fluorescent layer of nile blue (NB) that was further coated by another 5 nm of silica (SEF tags). The outer silica shell was then functionalized with antibodies to allow the detection of IgM as in typical immunological sandwich assays. IgM concentrations down to the 10 ng mL-1 mark were successfully detected. A linear dependence between the average fluorescence intensity and the IgM concentration was found.en
dc.description.affiliationSão Paulo State University (UNESP) School of Technology and Applied Sciences Campus Presidente Prudente
dc.description.affiliationUniversity of Victoria Department of Chemistry
dc.description.affiliationSão Paulo State University (UNESP) School of Sciences Humanities and Languages Campus Assis
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Technology and Applied Sciences Campus Presidente Prudente
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Sciences Humanities and Languages Campus Assis
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipTyö- ja Elinkeinoministeriö
dc.format.extent2717-2724
dc.identifierhttp://dx.doi.org/10.1039/c7an00639j
dc.identifier.citationAnalyst, v. 142, n. 15, p. 2717-2724, 2017.
dc.identifier.doi10.1039/c7an00639j
dc.identifier.issn1364-5528
dc.identifier.issn0003-2654
dc.identifier.lattes7384168674539702
dc.identifier.orcid0000-0003-4701-6408
dc.identifier.scopus2-s2.0-85025807026
dc.identifier.urihttp://hdl.handle.net/11449/174948
dc.language.isoeng
dc.relation.ispartofAnalyst
dc.relation.ispartofsjr1,249
dc.relation.ispartofsjr1,249
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleImmunoassay quantification using surface-enhanced fluorescence (SEF) tagsen
dc.typeArtigo
unesp.author.lattes7384168674539702[3]
unesp.author.lattes6118325967319836[4]
unesp.author.orcid0000-0003-4701-6408[3]
unesp.author.orcid0000-0002-5921-3161[4]

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