Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares

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dc.contributor.authorTongu, Eriky Akio de Oliveira [UNESP]
dc.contributor.authorSegabinazzi, Lorenzo G.T.M. [UNESP]
dc.contributor.authorAlvarenga, Marina Landim [UNESP]
dc.contributor.authorMonteiro, Aldine [UNESP]
dc.contributor.authorPapa, Frederico Ozanam [UNESP]
dc.contributor.authorAlvarenga, Marco Antonio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:58:22Z
dc.date.available2021-06-25T10:58:22Z
dc.date.issued2021-07-15
dc.description.abstractThis study aimed to evaluate the effects of mesenchymal stem cell-conditioned medium (MSC-CM) on sperm parameters, intrauterine polymorphonuclear neutrophils (PMN), intrauterine fluid accumulation (IUF), and fertility in mares. In experiment 1, two ejaculates from ten stallions were extended to 50 million sperm/mL using a milk-based extender. Thereafter, 20 mL of extended semen was added of MSC-CM as follows: 0, 5, 10, 15, and 20 mL. Sperm kinetics and plasma membrane integrity were evaluated immediately after dilution (T0) and 2 h post-incubation at 37 °C (T2). In experiment 2, mares characterized as resistant (n = 13) or susceptible (n = 7) to endometritis were inseminated with fresh semen 24 h post-induction of ovulation in two (Control and CM-1) and three (Control, CM-1, and CM-2) cycles in a crossover, as follows: control, no pharmacological interference; CM-1, supplementation of semen insemination dose at 3:4 (v:v, MSC-CM:semen); CM-2, 30 mL of MSC-CM was infused into the uterus 24 h before insemination. Endometrial cytology and uterine fluid were collected 6 and 24 h after insemination to evaluate the number of PMNs and concentrations of interleukins IL6, IL10, and TNFα. IUF was determined by ultrasonography 24 and 48 h after insemination. Pregnancy status was diagnosed 14 days after ovulation. The addition of MSC-CM to semen did not influence sperm parameters at T0 and T2 (P > 0.05) and reduced (CM-1; P < 0.05) the number of PMNs at 6 h post-insemination in resistant mares. In susceptible mares, PMNs at 6 and 24 h post-insemination, as well as IUF were reduced (P < 0.05) in both treated cycles (CM-1 and CM-2). In addition, MSC-CM downregulated IL6 and upregulated IL10 concentrations in the uterus of susceptible mares after insemination. There were no differences in fertility rates among groups both in resistant (Control, 77%, 10/13; CM-1, 62%, 8/13) and susceptible mares (Control, 42.8%, 3/7; CM-1, 57.1%, 4/7; CM-2, 85.7%. 6/7). In conclusion, MSC-CM did not affect sperm parameters when mixed with diluted semen, and reduced post-insemination inflammatory responses in mares.en
dc.description.affiliationDepartment of Veterinary Surgery and Animal Reproduction Faculty of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP
dc.description.affiliationUnespDepartment of Veterinary Surgery and Animal Reproduction Faculty of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2018/02856-3
dc.format.extent1-8
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2021.03.019
dc.identifier.citationTheriogenology, v. 169, p. 1-8.
dc.identifier.doi10.1016/j.theriogenology.2021.03.019
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85104473882
dc.identifier.urihttp://hdl.handle.net/11449/207628
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectCell-therapy
dc.subjectEndometritis
dc.subjectEquine
dc.subjectFertility
dc.subjectUterus
dc.titleAllogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in maresen
dc.typeArtigo

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