The Kallikrein Inhibitor from Bauhinia bauhinioides (BbKI) shows antithrombotic properties in venous and arterial thrombosis models

Página do item simplificado
dc.contributor.authorBrito, Marlon V.
dc.contributor.authorOliveira, Cleide de
dc.contributor.authorSalu, Bruno R.
dc.contributor.authorAndrade, Sonia A.
dc.contributor.authorMalloy, Paula M. D.
dc.contributor.authorSato, Ana C.
dc.contributor.authorVicente, Cristina P.
dc.contributor.authorSampaio, Misako U.
dc.contributor.authorMaffei, Francisco Humberto de Abreu [UNESP]
dc.contributor.authorOliva, Maria Luiza V.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:29Z
dc.date.available2014-12-03T13:10:29Z
dc.date.issued2014-05-01
dc.description.abstractThe Bauhinia bauhinioides Kallikrein Inhibitor (BbKI) is a Kunitz-type serine peptidase inhibitor of plant origin that has been shown to impair the viability of some tumor cells and to feature a potent inhibitory activity against human and rat plasma kallikrein (Kiapp 2.4 nmol/L and 5.2 nmol/L, respectively). This inhibitory activity is possibly responsible for an effect on hemostasis by prolonging activated partial thromboplastin time (aPTT). Because the association between cancer and thrombosis is well established, we evaluated the possible antithrombotic activity of this protein in venous and arterial thrombosis models. Vein thrombosis was studied in the vena cava ligature model in Wistar rats, and arterial thrombosis in the photochemical induced endothelium lesion model in the carotid artery of C57 black 6 mice. BbKI at a concentration of 2.0 mg/kg reduced the venous thrombus weight by 65% in treated rats in comparison to rats in the control group. The inhibitor prolonged the time for total artery occlusion in the carotid artery model mice indicating that this potent plasma kallikrein inhibitor prevented thrombosis. (C) 2014 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Bioquim & Biofis, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, BR-13083863 Campinas, SP, Brazil
dc.description.affiliationUNESP, Fac Med Botucatu, Dept Cirurgia & Ortopedia, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Botucatu, Dept Cirurgia & Ortopedia, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 09/53766-5
dc.description.sponsorshipIdFAPESP: 12/23640-2
dc.format.extent945-951
dc.identifierhttp://dx.doi.org/10.1016/j.thromres.2014.02.027
dc.identifier.citationThrombosis Research. Oxford: Pergamon-elsevier Science Ltd, v. 133, n. 5, p. 945-951, 2014.
dc.identifier.doi10.1016/j.thromres.2014.02.027
dc.identifier.issn0049-3848
dc.identifier.urihttp://hdl.handle.net/11449/112181
dc.identifier.wosWOS:000334739100038
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofThrombosis Research
dc.relation.ispartofjcr2.779
dc.relation.ispartofsjr1,096
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBlood coagulationen
dc.subjectKallikreinen
dc.subjectKunitz inhibitoren
dc.subjectThrombosisen
dc.subjectTrypsin inhibitoren
dc.subjectTumor cellsen
dc.titleThe Kallikrein Inhibitor from Bauhinia bauhinioides (BbKI) shows antithrombotic properties in venous and arterial thrombosis modelsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0001-9896-4630[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

Arquivos

Coleções

Artigos - Cirurgia e Ortopedia - FMB