Circulation of Different Lineages of Dengue Virus 2, Genotype American/Asian in Brazil: Dynamics and Molecular and Phylogenetic Characterization

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dc.contributor.authorDrumond, Betânia Paiva
dc.contributor.authorMondini, Adriano [UNESP]
dc.contributor.authorSchmidt, Diane J.
dc.contributor.authorde Bronzoni, Roberta Vieira Morais
dc.contributor.authorBosch, Irene
dc.contributor.authorNogueira, Maurício Lacerda
dc.contributor.institutionUniversidade Federal de Juiz de Fora (UFJF)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionTufts University
dc.contributor.institutionUniversidade Federal do Mato Grosso
dc.contributor.institutionMassachusetts Institute of Technology (MIT)
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.date.accessioned2014-05-27T11:28:42Z
dc.date.available2014-05-27T11:28:42Z
dc.date.issued2013-03-22
dc.description.abstractThe American/Asian genotype of Dengue virus type 2 (DENV-2) was introduced into the Americas in the 80′s. Although there is no data showing when this genotype was first introduced into Brazil, it was first detected in Brazil in 1990. After which the virus spread throughout the country and major epidemics occurred in 1998, 2007/08 and 2010. In this study we sequenced 12 DENV-2 genomes obtained from serum samples of patients with dengue fever residing in São José do Rio Preto, São Paulo (SJRP/SP), Brazil, in 2008. The whole open reading frame or envelope sequences were used to perform phylogenetic, phylogeographic and evolutionary analyses. Isolates from SJRP/SP were grouped within one lineage (BR3) close to isolates from Rio de Janeiro, Brazil. Isolates from SJRP were probably introduced there at least in 2007, prior to its detection in the 2008 outbreak. DENV-2 circulation in Brazil is characterized by the introduction, displacement and circulation of three well-defined lineages in different times, most probably from the Caribbean. Thirty-seven unique amino acid substitutions were observed among the lineages, including seven amino acid differences in domains I to III of the envelope protein. Moreover, we dated here, for the first time, the introduction of American/Asian genotype into Brazil (lineage BR1) to 1988/89, followed by the introduction of lineages BR2 (1998-2000) and BR3 (2003-05). Our results show a delay between the introduction and detection of DENV-2 lineages in Brazil, reinforcing the importance and need for surveillance programs to detect and trace the evolution of these viruses. Additionally, Brazilian DENV-2 differed in genetic diversity, date of introduction and geographic origin and distribution in Brazil, and these are important factors for the evolution, dynamics and control of dengue. © 2013 Drumond et al.en
dc.description.affiliationLaboratório de Virologia Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais
dc.description.affiliationLaboratório de Saúde Pública Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, São Paulo
dc.description.affiliationDepartment of Infectious Disease and Global Health Cummings School of Veterinary Medicine Tufts University, North Grafton, MA
dc.description.affiliationUniversidade Federal do Mato Grosso, Sinop, Mato Grosso
dc.description.affiliationGenome Resources in Dengue Consortium Massachusetts Institute of Technology, Cambridge, MA
dc.description.affiliationLaboratório de Pesquisa em Virologia Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo
dc.description.affiliationUnespLaboratório de Saúde Pública Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, São Paulo
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0059422
dc.identifier.citationPLoS ONE, v. 8, n. 3, 2013.
dc.identifier.doi10.1371/journal.pone.0059422
dc.identifier.file2-s2.0-84875331850.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes5821142624720398
dc.identifier.orcid0000-0002-5557-9721
dc.identifier.scopus2-s2.0-84875331850
dc.identifier.urihttp://hdl.handle.net/11449/74864
dc.identifier.wosWOS:000316549400061
dc.language.isoeng
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectvirus envelope protein
dc.subjectamino acid sequence
dc.subjectamino acid substitution
dc.subjectBrazil
dc.subjectcontrolled study
dc.subjectDengue virus 2
dc.subjectgene cluster
dc.subjectgenetic variability
dc.subjectgenome analysis
dc.subjectgeographic origin
dc.subjectgeographical variation (species)
dc.subjecthuman
dc.subjectmolecular evolution
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectopen reading frame
dc.subjectphylogenetic tree
dc.subjectphylogeny
dc.subjectphylogeography
dc.subjectstrain difference
dc.subjectvirus detection
dc.subjectvirus genome
dc.subjectvirus isolation
dc.subjectvirus strain
dc.subjectDengue Virus
dc.subjectGenetic Variation
dc.subjectGenome, Viral
dc.subjectGenotype
dc.subjectOpen Reading Frames
dc.subjectPhylogeny
dc.titleCirculation of Different Lineages of Dengue Virus 2, Genotype American/Asian in Brazil: Dynamics and Molecular and Phylogenetic Characterizationen
dc.typeArtigo
dcterms.licensehttp://www.plos.org/open-access/
unesp.author.lattes5821142624720398[2]
unesp.author.orcid0000-0002-5557-9721[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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