Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity

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dc.contributor.authorVeríssimo, Nathalia V. [UNESP]
dc.contributor.authorSaponi, Carolina F. [UNESP]
dc.contributor.authorRyan, Timothy M.
dc.contributor.authorGreaves, Tamar L.
dc.contributor.authorPereira, Jorge F.B. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionRMIT University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionAustralian Synchrotron
dc.contributor.institutionCIEPQPF
dc.date.accessioned2022-04-28T19:50:21Z
dc.date.available2022-04-28T19:50:21Z
dc.date.issued2021-12-01
dc.description.abstractFluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. Hence, the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial use. With this goal, we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based ([Cnmim]Cl) ionic liquids (ILs) as additives to preserve the activity of Enhanced Green Fluorescent Protein (EGFP) at different storage times and under unfavorable conditions. All [Cnmim]Cl solutions (at 0.100 mol L−1) were able to preserve EGFP fluorescence for longer than the phosphate-saline buffer (PBS) and NaCl solutions, increasing its fluorescence manifestation from 1 to 3 months. [Cnmim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence. [Cnmim]Cl also protected EGFP activity in the presence of the surfactant SDS, the acid guanidine hydrochloride, and H2O2. Therefore, [Cnmim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on EGFP. Therefore, there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions, and this is fundamental to enable expansion of FP in industrial and commercial applications.en
dc.description.affiliationDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01
dc.description.affiliationSchool of Science College of Science Engineering and Health RMIT University, 124 La Trobe Street
dc.description.affiliationSchool of Pharmaceutical Sciences São Paulo University (USP), Av. Prof. Lineu Prestes, no. 580, B16, 05508-000, Cidade de Universitária
dc.description.affiliationAustralian Synchrotron, 800 Blackburn Road
dc.description.affiliationUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima, Pólo II – Pinhal de Marrocos
dc.description.affiliationUnespDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipASCRS Research Foundation
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para a Ciência e a Tecnologia
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2014/16424–7
dc.description.sponsorshipIdFAPESP: 2014/19793–3
dc.description.sponsorshipIdFAPESP: 2016/07529–5
dc.description.sponsorshipIdFAPESP: 2018/01858–2
dc.description.sponsorshipIdFAPESP: 2018/06576–5
dc.description.sponsorshipIdFAPESP: 2018/20833–0
dc.description.sponsorshipIdFAPESP: 2018/25511–1
dc.description.sponsorshipIdFAPESP: 2018/50009–8
dc.description.sponsorshipIdFAPESP: 2020/14144–8
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: UIDB/EQU/00102/2020
dc.description.sponsorshipIdFundação para a Ciência e a Tecnologia: UIDP/EQU/00102/2020
dc.format.extent412-422
dc.identifierhttp://dx.doi.org/10.1016/j.gce.2021.08.001
dc.identifier.citationGreen Chemical Engineering, v. 2, n. 4, p. 412-422, 2021.
dc.identifier.doi10.1016/j.gce.2021.08.001
dc.identifier.issn2666-9528
dc.identifier.issn2096-9147
dc.identifier.scopus2-s2.0-85123982467
dc.identifier.urihttp://hdl.handle.net/11449/223399
dc.language.isoeng
dc.relation.ispartofGreen Chemical Engineering
dc.sourceScopus
dc.subjectFluorescence
dc.subjectGreen Fluorescent Protein
dc.subjectIonic liquids
dc.subjectPreservatives
dc.subjectProtein stability
dc.titleImidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activityen
dc.typeArtigo
unesp.author.orcid0000-0002-0298-0183[4]
unesp.author.orcid0000-0001-5959-0015 0000-0001-5959-0015[5]

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