New LncRNAs in Chronic Hepatitis C progression: from fibrosis to hepatocellular carcinoma

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dc.contributor.authorFerrasi, Adriana Camargo [UNESP]
dc.contributor.authorFernandez, Geysson Javier
dc.contributor.authorGrotto, Rejane Maria Tommasini [UNESP]
dc.contributor.authorSilva, Giovanni Faria [UNESP]
dc.contributor.authorGoncalves, Joao
dc.contributor.authorCosta, Marina C.
dc.contributor.authorEnguita, Francisco J.
dc.contributor.authorPardini, Maria Inês de Moura Campos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidad de Antioquia
dc.contributor.institutionFaculdade de Farmácia da Universidade de Lisboa
dc.contributor.institutionUniversidade de Lisboa
dc.date.accessioned2020-12-12T02:11:33Z
dc.date.available2020-12-12T02:11:33Z
dc.date.issued2020-12-01
dc.description.abstractHepatocellular carcinoma (HCC) is the third leading cause of cancer-related death in the world, and about 80% of the cases are associated with hepatitis B or C. Genetic and epigenetic alterations are accumulated over decades of chronic injury and may affect the functioning of tumor suppressor genes and protooncogenes. Studies have evidenced the role of Long non-coding RNAs (LncRNA) with oncogenic or tumor suppressor activities, suggesting a great potential in the treatment, diagnosis or indicator of prognosis in cancer. In this context, the aim of this study was to evaluate the global expression profile lncRNA in hepatic tissue samples with different stages of fibrosis associated with chronic hepatitis C, HCC and normal liver, in order to identify new lncRNAs that could contribute to study the progression of hepatic fibrosis to HCC associated with chronic hepatitis C. RNA-Seq was performed on Illumina NextSeq platform to identify lncRNAs expressed differently in 15 patients with chronic hepatitis C, three patients with HCC and three normal liver specimens. When the pathological tissues (fibrosis and carcinoma) were compared to normal hepatic tissue, were identified 2, 6 e 34 differentially expressed lncRNAs in moderate fibrosis, advanced fibrosis and HCC, respectively. The carcinoma group had the highest proportion of differentially expressed lncRNA (34) and of these, 29 were exclusive in this type of tissue. A heat map of the deregulated lncRNA revealed different expression patterns along the progression of fibrosis to HCC. The results showed the deregulation of some lncRNA already classified as tumor suppressors in HCC and other cancers, as well as some unpublished lncRNA whose function is unknown. Some of these lncRNAs are dysregulated since the early stages of liver injury in patients with hepatitis C, others overexpressed only in tumor tissue, indicating themselves as candidates of markers of fibrosis progression or tumor, with potential clinical applications in prognosis as well as a therapeutic target. Although there are already studies on lncRNA in hepatocellular carcinoma, this is the first study conducted in samples exclusively of HCV-related liver and HCV HCC.en
dc.description.affiliationDepartment of Internal Medicine Medical School Sao Paulo State University (UNESP)
dc.description.affiliationMolecular Biology Laboratory Blood Transfusion Center HC-FMB Sao Paulo State University (UNESP)
dc.description.affiliationGrupo Inmunovirologia Facultad de Medicina Universidad de Antioquia
dc.description.affiliationDepartment of Bioprocess and Biotechnology College of Agricultural Sciences Sao Paulo State University (UNESP)
dc.description.affiliationiMed-Research Institute of Medicines Faculdade de Farmácia da Universidade de Lisboa
dc.description.affiliationInstituto de Medicina Molecular João Lobo Antunes Faculdade de Medicina Universidade de Lisboa
dc.description.affiliationUnespDepartment of Internal Medicine Medical School Sao Paulo State University (UNESP)
dc.description.affiliationUnespMolecular Biology Laboratory Blood Transfusion Center HC-FMB Sao Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Bioprocess and Biotechnology College of Agricultural Sciences Sao Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1038/s41598-020-66881-2
dc.identifier.citationScientific Reports, v. 10, n. 1, 2020.
dc.identifier.doi10.1038/s41598-020-66881-2
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85086691090
dc.identifier.urihttp://hdl.handle.net/11449/200620
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.sourceScopus
dc.titleNew LncRNAs in Chronic Hepatitis C progression: from fibrosis to hepatocellular carcinomaen
dc.typeArtigo

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