Gymnotus coatesi (Gymnotiformes): A Case of Colocation of Multiple Sites of 18S rDNA with Telomeric Sequences

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dc.contributor.authorMachado, Milla de Andrade
dc.contributor.authorCardoso, Adauto Lima [UNESP]
dc.contributor.authorRodrigues Milhomem-Paixao, Susana Suely
dc.contributor.authorPieczarka, Julio Cesar
dc.contributor.authorNagamachi, Cleusa Yoshiko
dc.contributor.institutionUniv Fed Para
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Fed Educ Ciencia & Tecnol Goias
dc.date.accessioned2018-11-26T15:45:30Z
dc.date.available2018-11-26T15:45:30Z
dc.date.issued2017-10-01
dc.description.abstractGymnotus coatesi is a small and rare species of banded knife fish that was originally described by LaMonte in 1935, found along the main stretch of the Amazon River. There is no described cytogenetic data on this species. We analyzed the karyotype of five specimens of G. coatesi collected from Cururutuia Stream in Braganca, Para, Brazil. The obtained diploid number is 50 and the karyotypic formula is 24m/sm +26 st/a. The constitutive heterochromatin is DAPI positive and distributed mainly in the centromeric and pericentromeric regions of the chromosomes. Ag-nucleolus organizer regions staining showed nine active sites. The 5S rDNA probe hybridized chromosome pair 17 in the interstitial part of the long arm. Fluorescence in situ hybridization (FISH) with telomeric probes revealed signals only at terminal regions of the chromosomes. The 18S rDNA probe hybridized to 21 sites, and these signals colocalized with the telomeric sequences. This relatively high number of 18S rDNA sites may reflect gene duplication mediated by transposable elements. These results indicate that although the diploid number of G. coatesi is within the range previously observed for other members of the genus, various karyotypic characteristics distinguish G. coatesi from the other species of the genus and members of the Gymnotiform order.en
dc.description.affiliationUniv Fed Para, Inst Ciencias Biol, Ctr Estudos Avancados Biodiversidade, Lab Citogenet, Rua Augusto Correa 101, BR-66075110 Belem, Para, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Lab Genom Integrat, Sao Paulo, Brazil
dc.description.affiliationInst Fed Educ Ciencia & Tecnol Goias, Campus Valparaiso Goias, Goiania, Go, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Lab Genom Integrat, Sao Paulo, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBanco Nacional de Desenvolvimento Economico e Social-BNDES
dc.description.sponsorshipJCP
dc.description.sponsorshipCYN
dc.description.sponsorshipIdCNPq: 475013/2012-3
dc.description.sponsorshipIdCAPES: 047/2012 PRO-AMAZONIA
dc.description.sponsorshipIdFAPESP: TO 011/2008
dc.description.sponsorshipIdBanco Nacional de Desenvolvimento Economico e Social-BNDES: Operacao 2.318.698.0001
dc.description.sponsorshipIdJCP: 308401/2013-1
dc.description.sponsorshipIdCYN: 308428/2013-7
dc.format.extent459-463
dc.identifierhttp://dx.doi.org/10.1089/zeb.2017.1435
dc.identifier.citationZebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 5, p. 459-463, 2017.
dc.identifier.doi10.1089/zeb.2017.1435
dc.identifier.issn1545-8547
dc.identifier.urihttp://hdl.handle.net/11449/159855
dc.identifier.wosWOS:000412338600008
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofZebrafish
dc.relation.ispartofsjr0,795
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectchromosome
dc.subjectbanded knife fish
dc.subjectmultiple NOR
dc.subjectAmazon River
dc.titleGymnotus coatesi (Gymnotiformes): A Case of Colocation of Multiple Sites of 18S rDNA with Telomeric Sequencesen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc
unesp.author.orcid0000-0003-1516-2734[5]

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