Expression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasma

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dc.contributor.authorPerches, C. S. [UNESP]
dc.contributor.authorPellizzon, C. H. [UNESP]
dc.contributor.authorRanzani, J. J. T. [UNESP]
dc.contributor.authorDonatti, C.
dc.contributor.authorPadovani, C. R. [UNESP]
dc.contributor.authorMerlini, N. B. [UNESP]
dc.contributor.authorFonzar, J. F.
dc.contributor.authorBeserra, H. E. O. [UNESP]
dc.contributor.authorRocha, N. S. [UNESP]
dc.contributor.authorBrandao, C. V. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-27T06:00:14Z
dc.date.available2018-11-27T06:00:14Z
dc.date.issued2015-12-01
dc.description.abstractThe aim of this study was to evaluate the influence of platelet-rich (PRP) and poor (PPP) plasma in cell proliferation and matrix metalloproteinases (MMPs) expression during the repair of deep corneal ulcers. Forty-five female rabbits were distributed in 3 experimental groups (G) (n = 15), referred to as PRP (GR), PPP (GP) and Control (GC) groups, in accordance with the treatment. All animals underwent surgical induction of unilateral corneal ulcer. PRP and PPP eye drops were made by using centrifuged blood through standardized protocol, and instilled five times a day. In GC, lubricant eye drops were used. Each group was subdivided (n = 5) according to the final time point, 4 (M4), 7 (M7) and 30 days (M30). The animals' corneas were processed for morphological and immunohistochemical analysis for PCNA, MMP1, MMP2, MMP9, MT1-MMP and TIMP1. In M4, the levels of MMP2 were higher in GP and GR, and in M7, this behavior was only observed in the GP. In M30, more epithelial cells and MMP1 expression were found in GR than GP. In GR, cell proliferation was higher in M4 than at other time points and MMP2 expression was higher in M4 than M30. The PRP stimulates cell proliferation in the early phase (M4) of treatment when compared to other time points, different from other treatments. The use of eye drops of platelet-rich and poor plasma influences the expression of matrix metalloproteinases involved in the corneal repair process.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, Brazil
dc.format.extent1607-1615
dc.identifierhttp://dx.doi.org/10.1590/1678-4162-8142
dc.identifier.citationArquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 67, n. 6, p. 1607-1615, 2015.
dc.identifier.doi10.1590/1678-4162-8142
dc.identifier.fileS0102-09352015000601607.pdf
dc.identifier.issn0102-0935
dc.identifier.lattes0019393779801069
dc.identifier.lattes1087615389013655
dc.identifier.orcid0000-0002-4494-4180
dc.identifier.orcid0000-0002-2011-5214
dc.identifier.scieloS0102-09352015000601607
dc.identifier.urihttp://hdl.handle.net/11449/165012
dc.identifier.wosWOS:000367094600017
dc.language.isopor
dc.publisherArquivo Brasileiro Medicina Veterinaria Zootecnia
dc.relation.ispartofArquivo Brasileiro De Medicina Veterinaria E Zootecnia
dc.relation.ispartofsjr0,248
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectplatelet-poor plasma
dc.subjectMMP
dc.subjectPRP
dc.subjecthealing
dc.subjectcorneal
dc.titleExpression of matrix metalloproteinases and PCNA in deep corneal ulcers induced in rabbits, treated with platelet-rich plasmaen
dc.typeArtigo
dcterms.rightsHolderArquivo Brasileiro Medicina Veterinaria Zootecnia
unesp.author.lattes8727897080522289[5]
unesp.author.lattes0019393779801069[2]
unesp.author.lattes1087615389013655[10]
unesp.author.orcid0000-0002-7719-9682[5]
unesp.author.orcid0000-0002-4494-4180[2]
unesp.author.orcid0000-0002-2011-5214[10]

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