Impact of fipronil on the mushroom bodies of the stingless bee Scaptotrigona postica

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dc.contributor.authorJacob, Cynthia R. O. [UNESP]
dc.contributor.authorSoares, Hellen M. [UNESP]
dc.contributor.authorNocelli, Roberta C. F.
dc.contributor.authorMalaspina, Osmar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2015-03-18T15:52:35Z
dc.date.available2015-03-18T15:52:35Z
dc.date.issued2015-01-01
dc.description.abstractBACKGROUND: Studies on stingless bees are scarce, and little is known about these insects, especially regarding the effects of contamination by neurotoxic insecticides, which can cause damage to important structures of the insect brain. This study evaluated the morphological changes in the intrinsic neurons of the protocerebral mushroom bodies (Kenyon cells) of the stingless bee Scaptotrigona postica after exposure to different doses of fipronil, using light microscopy and transmission electron microscopy. This region of the brain was selected for analysis because of its importance as a sensory integration centre.RESULTS: In both oral and topical treatments, Kenyon cells presented pyknotic profiles, suggesting cell death. Statistical analysis showed significant differences among doses and exposure times. Transmission electron microscopy revealed changes in the nucleus and cellular organelles. Depending on the dose, the characteristics observed suggested apoptotosis or necrosis.CONCLUSION: This study demonstrates the toxic effects of fipronil. An increase in the number of pyknotic profiles of Kenyon cells of mushroom bodies was observed even at the sublethal doses of 0.27 ng AI bee(-1) and 0.24 ng AI mu L-1 in the topical and oral treatments respectively. Also, differences in the number of pyknotic profiles were dose and time dependent. (C) 2014 Society of Chemical Industryen
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho UNESP, Inst Biociencias, CEIS, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Ctr Ciencias Agr, Sao Paulo, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho UNESP, Inst Biociencias, CEIS, Sao Paulo, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 10/03723-5
dc.format.extent114-122
dc.identifierhttp://dx.doi.org/10.1002/ps.3776
dc.identifier.citationPest Management Science. Hoboken: Wiley-blackwell, v. 71, n. 1, p. 114-122, 2015.
dc.identifier.doi10.1002/ps.3776
dc.identifier.issn1526-498X
dc.identifier.lattes7538556085505819
dc.identifier.orcid0000-0002-1650-257X
dc.identifier.urihttp://hdl.handle.net/11449/116211
dc.identifier.wosWOS:000346034600015
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofPest Management Science
dc.relation.ispartofjcr3.249
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectapoptosisen
dc.subjectcytotoxicityen
dc.subjectphenylpyrazoleen
dc.subjectnecrosisen
dc.subjectnervous systemen
dc.titleImpact of fipronil on the mushroom bodies of the stingless bee Scaptotrigona posticaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes7538556085505819[4]
unesp.author.orcid0000-0002-1650-257X[4]

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Rio Claro - CEIS - Centro de Estudos de Insetos Sociais