Natural latex serum: characterization and biocompatibility assessment using Galleria mellonella as an alternative in vivo model

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dc.contributor.authorPegorin Brasil, Giovana Sant’Ana [UNESP]
dc.contributor.authorde Barros, Patrícia Pimentel [UNESP]
dc.contributor.authorMiranda, Matheus Carlos Romeiro
dc.contributor.authorde Barros, Natan Roberto
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorGomez, Alejandro
dc.contributor.authorHerculano, Rondinelli Donizetti [UNESP]
dc.contributor.authorde Mendonça, Ricardo José
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal University of Rio Grande do Norte (UFRN)
dc.contributor.institutionUniversity of Aveiro
dc.contributor.institutionTerasaki Institute for Biomedical Innovation (TIBI)
dc.contributor.institutionFederal University of Triangulo Mineiro (UFTM)
dc.date.accessioned2022-05-01T11:54:29Z
dc.date.available2022-05-01T11:54:29Z
dc.date.issued2022-01-01
dc.description.abstractNatural latex serum (NLS) is one of the natural rubber latex fractions from Hevea brasiliensis tree, which is formed by centrifuged serum and is composed of proteins, acids, nucleotides, salts and carbohydrates. The proteins present in NLS have demonstrated several interesting biological properties, including angiogenic, healing, osteogenic, anti-inflammatory, antimicrobial, in addition to inducing neovascularization, bone formation and osseointegration. Thus, we proposed to characterize NLS by physicochemical techniques and to investigate the biocompatibility by toxicological assays and safety test in Galleria mellonella. Infrared spectrum showed vibrational bands characteristic of amide I, II and III that are linked to the protein content, which was confirmed by the High Performance Liquid Chromatography profile and by the Electrophoresis analysis. This material did not exhibit hemolytic (rate <0.5%) and cytotoxic effects (viability >70%) and was able to enhance the proliferation of fibroblasts (>600%) after 3 days. The pronounced proliferative effect observed in fibroblast cells can be explained by the presence of the fibroblast growth factor (FGF) like protein revealed by the Western blot test. Moreover, NLS did not provoke toxic effects (survival ∼ 80%) on the G. mellonella model, indicating that it is a biocompatible and safe material.en
dc.description.affiliationInstitute of Chemistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationDepartment of Biotechnology and Bioprocess Engineering School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São Paulo
dc.description.affiliationMulticampi School of Medical Sciences Federal University of Rio Grande do Norte (UFRN), Rio Grande do Norte
dc.description.affiliationCentre for Environmental and Marine Studies (CESAM) University of Aveiro
dc.description.affiliationTerasaki Institute for Biomedical Innovation (TIBI)
dc.description.affiliationDepartment of Biochemistry Pharmacology and Physiology Federal University of Triangulo Mineiro (UFTM), Minas Gerais
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespDepartment of Biotechnology and Bioprocess Engineering School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São Paulo
dc.format.extent705-726
dc.identifierhttp://dx.doi.org/10.1080/09205063.2021.2014027
dc.identifier.citationJournal of Biomaterials Science, Polymer Edition, v. 33, n. 6, p. 705-726, 2022.
dc.identifier.doi10.1080/09205063.2021.2014027
dc.identifier.issn1568-5624
dc.identifier.issn0920-5063
dc.identifier.scopus2-s2.0-85122496702
dc.identifier.urihttp://hdl.handle.net/11449/233982
dc.language.isoeng
dc.relation.ispartofJournal of Biomaterials Science, Polymer Edition
dc.sourceScopus
dc.subjectGalleria mellonella
dc.subjectHevea brasiliensis
dc.subjectHPLC
dc.subjectNatural latex serum
dc.subjecttoxicity
dc.subjectWestern blot
dc.titleNatural latex serum: characterization and biocompatibility assessment using Galleria mellonella as an alternative in vivo modelen
dc.typeArtigo
unesp.author.orcid0000-0003-3817-8202[1]
unesp.author.orcid0000-0003-4885-2811[2]
unesp.author.orcid0000-0003-3881-2373[3]
unesp.author.orcid0000-0001-8689-4110[4]
unesp.author.orcid0000-0001-6646-6856[5]
unesp.author.orcid0000-0001-5171-890X[6]
unesp.author.orcid0000-0001-7236-0847[7]
unesp.author.orcid0000-0002-6957-3363[8]

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