Cheap assays of malate, glycerol and ethanol in fermenting must and food samples

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dc.contributor.authorGattas, EAL
dc.contributor.authorTininis, CRCS
dc.contributor.authorLauce, C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:30Z
dc.date.available2014-05-20T13:24:30Z
dc.date.issued2003-01-01
dc.description.abstractA preparation, enriched with malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glycerol -3- P dehydrogenase (GPDH) and glycerol kinase (GK), was obtained from dry baker's yeast. This preparation was used to assay glycerol, ethanol and malate measuring the variations in absorbance (NADH formation) at 340 nm. Good degrees of recoveries were obtained when glycerol was added to red wine and fermenting sugar-cane juice and when L-malate was added to commercial apple juice samples. Good results were also obtained when ethanol was assayed in fermented sugar-cane juice and wine samples, using both the partially purified preparation obtained from dry yeast and a purified commercial alcohol dehydrogenase.en
dc.description.affiliationUNESP, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, Brazil
dc.format.extent383-386
dc.identifier.citationEuromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine. 40128 Bologna: Medimond S R L, p. 383-386, 2003.
dc.identifier.urihttp://hdl.handle.net/11449/7614
dc.identifier.wosWOS:000184180200070
dc.language.isoeng
dc.publisherMedimond S R L
dc.relation.ispartofEuromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdry baker's yeastpt
dc.subjectglycerolpt
dc.subjectethanolpt
dc.subjectmalate assaypt
dc.titleCheap assays of malate, glycerol and ethanol in fermenting must and food samplesen
dc.typeTrabalho apresentado em evento
dcterms.rightsHolderMedimond S R L
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Alimentos e Nutrição - FCFAR