Differential arthritogenicity of Staphylococcus aureus strains isolated from biological samples

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dc.contributor.authorColavite-Machado, Priscila M. [UNESP]
dc.contributor.authorIshikawa, Larissa Lumi W. [UNESP]
dc.contributor.authorDonegá França, Thaís G. [UNESP]
dc.contributor.authorZorzella-Pezavento, Sofia Fernanda G. [UNESP]
dc.contributor.authorda Rosa, Larissa C. [UNESP]
dc.contributor.authorChiuso-Minicucci, Fernanda [UNESP]
dc.contributor.authorda Cunha, Maria de Lourdes Ribeiro de Souza [UNESP]
dc.contributor.authorGarlet, Gustavo P.
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:30:30Z
dc.date.available2014-05-27T11:30:30Z
dc.date.issued2013-08-30
dc.description.abstractBackground: Staphylococcus aureus is the most common agent of septic arthritis that is a severe, rapidly progressive and destructive joint disease. Superantigens produced by S. aureus are considered the major arthritogenic factors. In this study, we compared the arthritogenic potential of five superantigen-producing staphylococcal strains.Methods: Male C57BL/6 mice were intravenously infected with ATCC 19095 SEC+, N315 ST5 TSST-1+, S-70 TSST-1+, ATCC 51650 TSST-1+ and ATCC 13565 SEA+ strains. Clinical parameters as body weight, arthritis incidence and clinical score were daily evaluated. Joint histopathological analysis and spleen cytokine production were evaluated at the 14th day after infection.Results: Weight loss was observed in all infected mice. ATCC 19095 SEC+, N315 ST5 TSST-1+ and S-70 TSST-1+ were arthritogenic, being the highest scores observed in ATCC 19095 SEC+ infected mice. Intermediate and lower clinical scores were observed in N315 ST5 TSST-1+ and S-70 TSST-1+ infected mice, respectively. The ATCC 13565 SEA+ strain caused death of 85% of the animals after 48 h. Arthritis triggered by the ATCC 19095 SEC+ strain was characterized by accentuated synovial hyperplasia, inflammation, pannus formation, cartilage destruction and bone erosion. Similar joint alterations were found in N315 ST5 TSST-1+ infected mice, however they were strikingly more discrete. Only minor synovial proliferation and inflammation were triggered by the S-70 TSST-1+ strain. The lowest levels of TNF-α, IL-6 and IL-17 production in response to S. aureus stimulation were found in cultures from mice infected with the less arthritogenic strains (S-70 TSST-1+ and ATCC 51650 TSST-1+). The highest production of IL-17 was detected in mice infected with the most arthritogenic strains (ATCC 19095 SEC+ and N315 ST5 TSST-1+).Conclusions: Together these results demonstrated that S. aureus strains, isolated from biological samples, were able to induce a typical septic arthritis in mice. These results also suggest that the variable arthritogenicity of these strains was, at least in part, related to their differential ability to induce IL-17 production. © 2013 Colavite-Machado et al.; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Microbiology and Immunology Biosciences Institute Univ. Estadual Paulista (UNESP), Distrito de Rubião Júnior s/n, 18618-070 Botucatu, São Paulo
dc.description.affiliationDepartment of Biological Sciences School of Dentistry of Bauru São Paulo University-FOB/USP, Bauru, São Paulo
dc.description.affiliationUnespDepartment of Microbiology and Immunology Biosciences Institute Univ. Estadual Paulista (UNESP), Distrito de Rubião Júnior s/n, 18618-070 Botucatu, São Paulo
dc.identifierhttp://dx.doi.org/10.1186/1471-2334-13-400
dc.identifier.citationBMC Infectious Diseases, v. 13, n. 1, 2013.
dc.identifier.doi10.1186/1471-2334-13-400
dc.identifier.file2-s2.0-84884958822.pdf
dc.identifier.issn1471-2334
dc.identifier.lattes4977572416129527
dc.identifier.scopus2-s2.0-84884958822
dc.identifier.urihttp://hdl.handle.net/11449/76365
dc.identifier.wosWOS:000324262500001
dc.language.isoeng
dc.relation.ispartofBMC Infectious Diseases
dc.relation.ispartofjcr2.620
dc.relation.ispartofsjr1,576
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectIL-17
dc.subjectSeptic arthritis
dc.subjectStaphylococcus aureus
dc.titleDifferential arthritogenicity of Staphylococcus aureus strains isolated from biological samplesen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes4977572416129527[9]
unesp.author.orcid0000-0002-5071-8382[8]
unesp.author.orcid0000-0003-4557-3331[9]
unesp.author.orcid0000-0001-9030-0768[4]
unesp.author.orcid0000-0003-3481-2181[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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