Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

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dc.contributor.authorZaros, Lilian Giotto
dc.contributor.authorBricarello, Patrízia Ana [UNESP]
dc.contributor.authorAmarante, Alessandro Francisco Talamini do [UNESP]
dc.contributor.authorCoutinho, Luiz Lehmann
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:52:56Z
dc.date.available2014-05-20T13:52:56Z
dc.date.issued2007-01-01
dc.description.abstractT cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.en
dc.description.affiliationUniversidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Zootecnia
dc.description.affiliationUniversidade Estadual Paulista Departamento de Parasitologia
dc.description.affiliationUnespUniversidade Estadual Paulista Departamento de Parasitologia
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent575-579
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572007000400012
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 575-579, 2007.
dc.identifier.doi10.1590/S1415-47572007000400012
dc.identifier.fileS1415-47572007000400012.pdf
dc.identifier.issn1415-4757
dc.identifier.lattes2677231663329706
dc.identifier.scieloS1415-47572007000400012
dc.identifier.urihttp://hdl.handle.net/11449/18890
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectcytokine gene expressionen
dc.subjectreal-time RT-PCRen
dc.subjectcattleen
dc.subjectgastrointestinal nematodesen
dc.subjectbovine cytokinesen
dc.titleQuantification of bovine cytokine gene expression using real-time RT-PCR methodologyen
dc.typeArtigo
unesp.author.lattes2677231663329706[3]
unesp.author.orcid0000-0003-3995-5501[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Parasitologia - IBB