In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair

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dc.contributor.authorMendes, Pericles Nobrega [UNESP]
dc.contributor.authorRahal, Sheila Canevese [UNESP]
dc.contributor.authorMarques Pereira-Junior, Oduvaldo Camara [UNESP]
dc.contributor.authorFabris, Viciany Erique [UNESP]
dc.contributor.authorRahal Lenharo, Sara Lais
dc.contributor.authorde Lima-Neto, Joao Ferreira [UNESP]
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDept Fed Police
dc.date.accessioned2014-05-20T13:37:22Z
dc.date.available2014-05-20T13:37:22Z
dc.date.issued2009-03-24
dc.description.abstractBackground: Barrier materials as cellulose membranes are used for guided tissue repair. However, it is essential that the surrounding tissues accept the device. The present study histologically evaluated tissue reaction to a microbial cellulose membrane after subcutaneous implantation in mice. Furthermore, the interaction between mesenchymal stem cells and the biomaterial was studied in vitro to evaluate its ability to act as cellular scaffold for tissue engineering.Methods: Twenty-five Swiss Albino mice were used. A 10 x 10 mm cellulose membrane obtained through biosynthesis using Acetobacter xylinum bacteria was implanted into the lumbar subcutaneous tissue of each mouse. The mice were euthanatized at seven, 15, 30, 60, and 90 days, and the membrane and surrounding tissues were collected and examined by histology.Results: A mild inflammatory response without foreign body reaction was observed until 30 days post-surgery around the implanted membrane. Polarized microscopy revealed that the membrane remained intact at all evaluation points. Scanning electron microscopy of the cellulose membrane surface showed absence of pores. The in vitro evaluation of the interaction between cells and biomaterial was performed through viability staining analysis of the cells over the biomaterial, which showed that 95% of the mesenchymal stem cells aggregating to the cellulose membrane were alive and that 5% were necrotic. Scanning electron microscopy showed mesenchymal stem cells with normal morphology and attached to the cellulose membrane surface.Conclusion: The microbial cellulose membrane evaluated was found to be nonresorbable, induced a mild inflammatory response and may prove useful as a scaffold for mesenchymal stem cells.en
dc.description.affiliationUNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, Botucatu, SP, Brazil
dc.description.affiliationUNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationDept Fed Police, Brasilia, DF, Brazil
dc.description.affiliationUnespUNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP, Brazil
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1186/1751-0147-51-12
dc.identifier.citationActa Veterinaria Scandinavica. London: Biomed Central Ltd., v. 51, p. 8, 2009.
dc.identifier.doi10.1186/1751-0147-51-12
dc.identifier.fileWOS000265534300001.pdf
dc.identifier.issn0044-605X
dc.identifier.lattes1497433265390194
dc.identifier.lattes4880803690413862
dc.identifier.orcid0000-0002-9211-4093
dc.identifier.urihttp://hdl.handle.net/11449/12921
dc.identifier.wosWOS:000265534300001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofActa Veterinaria Scandinavica
dc.relation.ispartofjcr1.497
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleIn vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repairen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes1497433265390194
unesp.author.lattes4880803690413862
unesp.author.orcid0000-0002-9211-4093[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ
Artigos - Patologia - FMB