Genome-wide analysis of EgEVE_1, a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomes

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dc.contributor.authorMarcon, Helena Sanches [UNESP]
dc.contributor.authorCosta-Silva, Juliana
dc.contributor.authorLorenzetti, Alan Péricles Rodrigues
dc.contributor.authorMarino, Celso Luis [UNESP]
dc.contributor.authorDomingues, Douglas Silva [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Tecnológica Federal do Paraná
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2018-12-11T17:32:14Z
dc.date.available2018-12-11T17:32:14Z
dc.date.issued2017-01-01
dc.description.abstractEndogenous viral elements (EVEs) are the result of heritable horizontal gene transfer from viruses to hosts. In the last years, several EVE integration events were reported in plants by the exponential availability of sequenced genomes. Eucalyptus grandis is a forest tree species with a sequenced genome that is poorly studied in terms of evolution and mobile genetic elements composition. Here we report the characterization of E. grandis endogenous viral element 1 (EgEVE_1), a transcriptionally active EVE with a size of 5,664 bp. Phylogenetic analysis and genomic distribution demonstrated that EgEVE_1 is a newly described member of the Caulimoviridae family, distinct from the recently characterized plant Florendoviruses. Genomic distribution of EgEVE_1 and Florendovirus is also distinct. EgEVE_1 qPCR quantification in Eucalyptus urophylla suggests that this genome has moreEgEVE_1 copies than E. grandis. EgEVE_1 transcriptional activity was demonstrated by RT-qPCR in five Eucalyptus species and one intrageneric hybrid. We also identified that Eucalyptus EVEs can generate small RNAs (sRNAs),that might be involved in de novo DNA methylation and virus resistance. Our data suggest that EVE families in Eucalyptus have distinct properties, and we provide the first comparative analysis of EVEs in Eucalyptus genomes.en
dc.description.affiliationDepartamento de Botânica Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
dc.description.affiliationDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
dc.description.affiliationPPGBIOINFO Universidade Tecnológica Federal do Paraná
dc.description.affiliationGenética e Biologia Molecular Universidade Estadual de Londrina
dc.description.affiliationUnespDepartamento de Botânica Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
dc.description.affiliationUnespDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 474123/2010-3
dc.format.extent217-225
dc.identifierhttp://dx.doi.org/10.1590/1678-4685-gmb-2016-0086
dc.identifier.citationGenetics and Molecular Biology, v. 40, n. 1, p. 217-225, 2017.
dc.identifier.doi10.1590/1678-4685-gmb-2016-0086
dc.identifier.fileS1415-47572017000200217.pdf
dc.identifier.issn1678-4685
dc.identifier.issn1415-4757
dc.identifier.lattes0165348738208319
dc.identifier.orcid0000-0003-4524-954X
dc.identifier.scieloS1415-47572017000200217
dc.identifier.scopus2-s2.0-85018287246
dc.identifier.urihttp://hdl.handle.net/11449/178820
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCaulimovirus
dc.subjectEucalyptus
dc.subjectHorizontal transfer
dc.subjectInsertion
dc.subjectPararetrovirus
dc.titleGenome-wide analysis of EgEVE_1, a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomesen
dc.typeArtigo
unesp.author.lattes0165348738208319[4]
unesp.author.orcid0000-0003-4524-954X[4]

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