Role of TLR-2 and fungal surface antigens on innate immune response against Sporothrix schenckii

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dc.contributor.authorNegrini, Thais de C. [UNESP]
dc.contributor.authorFerreira, Lucas S. [UNESP]
dc.contributor.authorAlegranci, Pâmela [UNESP]
dc.contributor.authorArthur, Rodrigo A.
dc.contributor.authorSundfeld, Pedro P. [UNESP]
dc.contributor.authorMaia, Danielle C. G. [UNESP]
dc.contributor.authorSpolidório, Luis Carlos [UNESP]
dc.contributor.authorCarlos, Iracilda Z. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSchool of Dentistry
dc.contributor.institutionFederal University of Rio Grande do Sul
dc.date.accessioned2014-05-27T11:27:32Z
dc.date.available2014-05-27T11:27:32Z
dc.date.issued2013-01-01
dc.description.abstractSporotrichosis is an infection caused by the dimorphic fungus Sporothrix schenckii. Toll-like receptors (TLRs) play an important role in immunity, since they bind to pathogen surface antigens and initiate the immune response. However, little is known about the role of TLR-2 and fungal surface antigens in the recognition of S. schenckii and in the subsequent immune response. This study aimed to evaluate the involvement of TLR-2 and fungal surface soluble (SolAg) and lipidic (LipAg) antigens in phagocytosis of S. schenckii and production of immune mediators by macrophages obtained from WT and TLR-2 -/- animals. The results showed that TLR-2-/- animals had had statistical lower percentage of macrophages with internalized yeasts compared to WT. SolAg and LipAg impaired phagocytosis and immunological mediator production for both WT and TLR-2-/-. The absence of TLR-2 led to lower production of the cytokines TNF, IL-1β, IL-12 and IL-10 compared to WT animals. These results suggest a new insight in relation to how the immune system, through TLR-2, recognizes and induces the production of mediators in response to the fungus S. schenckii. Copyright © Informa Healthcare USA, Inc.en
dc.description.affiliationDepartment of Clinical Analysis Araraquara School of Pharmaceutical Sciences Sao Paulo State University, Rua Expedicionários do Brasil, No. 1621, Araraquara, SP, CEP 14.801-902
dc.description.affiliationDepartment of Preventive and Community Dentistry Indiana University School of Dentistry, Indianapolis, IN
dc.description.affiliationDepartment of Preventive and Community Dentistry School of Dentistry Federal University of Rio Grande do Sul, RS
dc.description.affiliationDepartment of Physiology and Pathology Araraquara School of Dentistry São Paulo State University, SP
dc.description.affiliationUnespDepartment of Clinical Analysis Araraquara School of Pharmaceutical Sciences Sao Paulo State University, Rua Expedicionários do Brasil, No. 1621, Araraquara, SP, CEP 14.801-902
dc.description.affiliationUnespDepartment of Physiology and Pathology Araraquara School of Dentistry São Paulo State University, SP
dc.format.extent36-48
dc.identifierhttp://dx.doi.org/10.3109/08820139.2012.719982
dc.identifier.citationImmunological Investigations, v. 42, n. 1, p. 36-48, 2013.
dc.identifier.doi10.3109/08820139.2012.719982
dc.identifier.issn0882-0139
dc.identifier.issn1532-4311
dc.identifier.lattes2640929291808415
dc.identifier.scopus2-s2.0-84870950102
dc.identifier.urihttp://hdl.handle.net/11449/74285
dc.identifier.wosWOS:000312224700003
dc.language.isoeng
dc.relation.ispartofImmunological Investigations
dc.relation.ispartofjcr2.588
dc.relation.ispartofsjr0,740
dc.relation.ispartofsjr0,740
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCytokines
dc.subjectMacrophages
dc.subjectMice
dc.subjectPhagocytosis
dc.subjectSporothrix schenckii
dc.subjectTLR-2
dc.subjectfungal lipidic antigen
dc.subjectfungal surface soluble antigen
dc.subjectfungus antigen
dc.subjectinterleukin 10
dc.subjectinterleukin 12
dc.subjectinterleukin 1beta
dc.subjecttoll like receptor 2
dc.subjecttumor necrosis factor alpha
dc.subjectunclassified drug
dc.subjectanimal experiment
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectfemale
dc.subjectinnate immunity
dc.subjectmacrophage
dc.subjectmouse
dc.subjectnonhuman
dc.subjectphagocytosis
dc.subjectpriority journal
dc.subjectAnimals
dc.subjectAntigens, Fungal
dc.subjectCells, Cultured
dc.subjectFemale
dc.subjectImmunity, Innate
dc.subjectInflammation Mediators
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectSporothrix
dc.subjectSporotrichosis
dc.subjectToll-Like Receptor 2
dc.titleRole of TLR-2 and fungal surface antigens on innate immune response against Sporothrix schenckiien
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
unesp.author.lattes2640929291808415
unesp.author.orcid0000-0003-2282-7562[2]
unesp.author.orcid0000-0002-0084-3468[8]
unesp.author.orcid0000-0002-0592-542X[7]
unesp.author.orcid0000-0001-8107-413X[4]
unesp.author.orcid0000-0003-4064-0101[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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