Antimicrobial resistance and persistence of Staphylococcus epidermidis clones in a Brazilian university hospital

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dc.contributor.authorMartins, André [UNESP]
dc.contributor.authorRiboli, Danilo Flávio Moraes [UNESP]
dc.contributor.authorCamargo, Carlos Henrique [UNESP]
dc.contributor.authorPereira, Valéria Cataneli [UNESP]
dc.contributor.authorDe Almeida Sampaio, Rogério [UNESP]
dc.contributor.authorCunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:46Z
dc.date.available2014-05-27T11:30:46Z
dc.date.issued2013-10-01
dc.description.abstractOxacillin is an alternative for the treatment of Staphylococcus spp. infections; however, resistance to this drug has become a major problem over recent decades. The main objective of this study was to epidemiologically characterize coagulase-negative staphylococci (CoNS) strains recovered from blood of patients hospitalized in a Brazilian teaching hospital. Oxacillin resistance was analyzed in 160 strains isolated from blood culture samples by phenotypic methods, detection of the mecA gene, and determination of intermediate sensitivity to vancomycin on brain heart infusion agar supplemented with 4 and 6 μg/mL vancomycin. In addition, characterization of the epidemiological profile by staphylococcal cassette chromosome mec (SCC. mec) typing and clonal analysis by pulsed-field gel electrophoresis (PFGE) were performed. The mecA gene was detected in 72.5% of the isolates. Methicillin-resistant CoNS isolates exhibited the highest minimum inhibitory concentrations and multiresistance when compared to methicillin-susceptible CoNS strains. Typing classified 32.8% of the isolates as SCC. mec I and 50% as SCC. mec III. PFGE typing of the SCC. mec III Staphylococcus epidermidis isolates identified 6 clones disseminated in different wards that persisted from 2002 to 2009. The high oxacillin resistance rates found in this study and clonal dissemination in different wards highlight the importance of good practices in nosocomial infection control and of the rational use of antibiotic therapy in order to prevent the dissemination of these clones. © 2013 Elsevier Inc.en
dc.description.affiliationDepartment of Tropical Diseases and Diagnostic Imaging Botucatu Medical School Universidade Estadual Paulista (UNESP), City of Botucatu
dc.description.affiliationDepartment of Microbiology and Immunology Biosciences Institute Universidade Estadual Paulista (UNESP), City of Botucatu
dc.description.affiliationDepartment of Biostatistics Biosciences Institute Universidade Estadual Paulista (UNESP), City of Botucatu
dc.description.affiliationUnespDepartment of Tropical Diseases and Diagnostic Imaging Botucatu Medical School Universidade Estadual Paulista (UNESP), City of Botucatu
dc.description.affiliationUnespDepartment of Microbiology and Immunology Biosciences Institute Universidade Estadual Paulista (UNESP), City of Botucatu
dc.description.affiliationUnespDepartment of Biostatistics Biosciences Institute Universidade Estadual Paulista (UNESP), City of Botucatu
dc.format.extent164-168
dc.identifierhttp://dx.doi.org/10.1016/j.diagmicrobio.2013.06.005
dc.identifier.citationDiagnostic Microbiology and Infectious Disease, v. 77, n. 2, p. 164-168, 2013.
dc.identifier.doi10.1016/j.diagmicrobio.2013.06.005
dc.identifier.issn0732-8893
dc.identifier.issn1879-0070
dc.identifier.lattes0115647772315973
dc.identifier.scopus2-s2.0-84884414718
dc.identifier.urihttp://hdl.handle.net/11449/76704
dc.identifier.wosWOS:000325449100016
dc.language.isoeng
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
dc.relation.ispartofjcr2.341
dc.relation.ispartofsjr1,157
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectClones
dc.subjectCoNS
dc.subjectOxacillin resistance
dc.subjectSCCmec
dc.subjectStaphylococcus epidermidis
dc.titleAntimicrobial resistance and persistence of Staphylococcus epidermidis clones in a Brazilian university hospitalen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes0115647772315973
unesp.author.orcid0000-0002-2545-5824[2]
unesp.author.orcid0000-0001-6834-0085[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
Artigos - Bioestatística - IBB
Artigos - Microbiologia e Imunologia - IBB