Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering

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dc.contributor.authorClaro, Amanda Maria
dc.contributor.authorAlves, Caroline Cássia
dc.contributor.authordos Santos, Kelvin Sousa [UNESP]
dc.contributor.authorda Rocha, Euzane Gomes
dc.contributor.authorde Lima Fontes, Marina
dc.contributor.authorMonteiro, Gustavo Claro
dc.contributor.authorde Carvalho, Gustavo Senra Gonçalves
dc.contributor.authorCaiut, José Maurício Almeida
dc.contributor.authorMoroz, Andrei [UNESP]
dc.contributor.authorRibeiro, Sidney José Lima [UNESP]
dc.contributor.authorS. Barud, Hernane
dc.contributor.institutionUniversity of Araraquara—UNIARA
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:35:01Z
dc.date.available2023-07-29T13:35:01Z
dc.date.issued2023-07-01
dc.description.abstractTissue engineering has emerged as a multidisciplinary field that aims to improve health and quality of life by restoring functions of tissues and organs. Cells and scaffolds are the two major components of tissue engineering. Scaffolds act as a support for cells, thus facilitating cell adhesion, proliferation, morphogenesis, differentiation, and extracellular matrix production. Since three-dimensional (3D) porous scaffolds can better simulate the native 3D architecture of in vivo systems than conventional 2D cultures, they are more appropriate to support tissue regeneration. This study aimed to use regenerated cellulose sponge (RCS) as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold (ASS). RCS was coated with boehmite-GPTS (3-glycidoxypropyltrimethoxysilane) solution and dried at 60 °C for 12 h. The coated RCS was converted into porous alumina-silica scaffold via thermal treatment at 500 °C for 4 h in air. The materials were characterized by Fourier transform‐infrared (FT‐IR), X-Ray diffraction (XRD), thermogravimetric analysis (TGA) aluminum-27 and silicon-29 nuclear magnetic resonance (NMR), scanning electron microscopy, and energy dispersive X-ray spectroscopy (SEM-EDS). The MTT metabolism assays were used to evaluate indirectly cytocompatibility and cell proliferation using MCT3T3-E1, HDFa and HaCaT cells. The biological in vitro assays demonstrated that only RCS exhibited toxicity toward HDFa cells, although this behavior has been shown questionable once other studies have reported the non-cytotoxic, mutagenic and genotoxic potential of RCS. We believe that tests including clonogenic and mutagenic assays should be performed using HDFa cells in contact with RCS-derived extract in order to further investigate this behavior. The sponge materials have demonstrated different growth rate and adhesion to three different cell lines evaluated. Therefore, the employed sacrificial template approach presents as a viable alternative to produce 3D scaffolds for tissue engineering. Graphical abstract: [Figure not available: see fulltext.]en
dc.description.affiliationUniversity of Araraquara—UNIARA, Rua Carlos Gomes 1217, SP
dc.description.affiliationDepartment of Chemistry Faculdade de Filosofia Ciências e Letras de Ribeirão Preto University of São Paulo, SP
dc.description.affiliationThe School of Pharmaceutical Sciences—FCF Department of Clinical Analysis São Paulo State University—UNESP, Rodovia Araraquara-Jaú, KM 01, SP
dc.description.affiliationInstitute of Chemistry São Paulo State University—UNESP, Rua Prof. Francisco Degni 55, SP
dc.description.affiliationUnespThe School of Pharmaceutical Sciences—FCF Department of Clinical Analysis São Paulo State University—UNESP, Rodovia Araraquara-Jaú, KM 01, SP
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University—UNESP, Rua Prof. Francisco Degni 55, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent83-95
dc.identifierhttp://dx.doi.org/10.1007/s10971-022-05990-y
dc.identifier.citationJournal of Sol-Gel Science and Technology, v. 107, n. 1, p. 83-95, 2023.
dc.identifier.doi10.1007/s10971-022-05990-y
dc.identifier.issn1573-4846
dc.identifier.issn0928-0707
dc.identifier.scopus2-s2.0-85145327268
dc.identifier.urihttp://hdl.handle.net/11449/248119
dc.language.isoeng
dc.relation.ispartofJournal of Sol-Gel Science and Technology
dc.sourceScopus
dc.subjectAlumina-silica
dc.subjectSacrificial template
dc.subjectScaffold
dc.subjectTissue engineering
dc.titleRegenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineeringen
dc.typeArtigo
unesp.author.orcid0000-0003-0076-7298[1]
unesp.author.orcid0000-0002-7397-5904[4]
unesp.author.orcid0000-0003-2984-9561[5]
unesp.author.orcid0000-0002-3011-3028[6]
unesp.author.orcid0000-0003-1632-8070[7]
unesp.author.orcid0000-0002-3161-5302[8]
unesp.author.orcid0000-0002-4498-9784[9]
unesp.author.orcid0000-0003-3286-9440[10]
unesp.author.orcid0000-0001-9081-2413[11]

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