Histochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training

Página do item simplificado
dc.contributor.authorRemedio, R. N. [UNESP]
dc.contributor.authorBarbosa, R. A. [UNESP]
dc.contributor.authorCastellar, A. [UNESP]
dc.contributor.authorGomes, R. J.
dc.contributor.authorCaetano, F. H. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-27T11:25:57Z
dc.date.available2014-05-27T11:25:57Z
dc.date.issued2011-08-01
dc.description.abstractAlterations in liver functions are common among diabetic patients, and many symptoms in the liver have been reported, including changes in glycogen stores and in the amount of collagen fibers. The practice of physical training and its morphological effects in this organ, however, are scarcely studied. In order to observe the morphological effects of alloxan-induced diabetes and the alterations arising from the practice of long-term chronic physical training in the liver, samples were collected and processed, and then analyzed by means of the histochemical techniques Periodic Acid-Schiff and Picrosirius-Hematoxylin, and ultrastructural cytochemical test of Afzelius. Through evaluation of the tissue, it was observed a drastic reduction in hepatic glycogen stores of sedentary diabetics, recovered in trained diabetic rats. Furthermore, it was detected a decrease in the content of perisinusoidal collagen fibers in the diabetic liver, also recovered due to the development of a training protocol. On ultrastructural level, cytochemical analysis confirmed the loss of glycogen and the recovery obtained by training. In conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. © 2011 Elsevier Ltd.en
dc.description.affiliationDepartment of Biology São Paulo State University (UNESP), Rio Claro, SP
dc.description.affiliationDepartment of Biosciences São Paulo Federal University (UNIFESP), Santos, SP
dc.description.affiliationUnespDepartment of Biology São Paulo State University (UNESP), Rio Claro, SP
dc.format.extent207-215
dc.identifierhttp://dx.doi.org/10.1016/j.tice.2011.03.003
dc.identifier.citationTissue and Cell, v. 43, n. 4, p. 207-215, 2011.
dc.identifier.doi10.1016/j.tice.2011.03.003
dc.identifier.issn0040-8166
dc.identifier.issn1532-3072
dc.identifier.lattes4396826019535898
dc.identifier.scopus2-s2.0-79960182170
dc.identifier.urihttp://hdl.handle.net/11449/72571
dc.language.isoeng
dc.relation.ispartofTissue and Cell
dc.relation.ispartofjcr1.438
dc.relation.ispartofsjr0,410
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDiabetes
dc.subjectHistochemistry
dc.subjectLiver
dc.subjectPhysical training
dc.subjectUltrastructure
dc.subjectcollagen fiber
dc.subjectglycogen
dc.subjectaerobic exercise
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcontrolled study
dc.subjectcytochemistry
dc.subjectdiabetes mellitus
dc.subjectglycogen analysis
dc.subjectglycogen liver level
dc.subjecthistochemistry
dc.subjectliver fibrosis
dc.subjectliver sinusoid
dc.subjectlong term care
dc.subjectmale
dc.subjectnonhuman
dc.subjectperiodic acid Schiff stain
dc.subjectrat
dc.subjecttraining
dc.subjectultrastructure
dc.subjectAnimals
dc.subjectBlood Glucose
dc.subjectCollagen
dc.subjectDiabetes Mellitus, Experimental
dc.subjectExtracellular Matrix
dc.subjectInsulin
dc.subjectInsulin-Like Growth Factor I
dc.subjectLiver Glycogen
dc.subjectMale
dc.subjectPhysical Conditioning, Animal
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRattus
dc.titleHistochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical trainingen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes4396826019535898
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

Arquivos

Coleções

Artigos - Biologia - IBRC