Functionality of the Interleukin 8 haplotypes in lymphocytes and macrophages in response to gram-negative periodontopathogens

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dc.contributor.authorPigossi, Suzane C. [UNESP]
dc.contributor.authorAnovazzi, Giovana [UNESP]
dc.contributor.authorFinoti, Livia S. [UNESP]
dc.contributor.authorde Medeiros, Marcell C. [UNESP]
dc.contributor.authorMayer, Marcia P.A.
dc.contributor.authorRossa Junior, Carlos [UNESP]
dc.contributor.authorScarel-Caminaga, Raquel M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T16:11:03Z
dc.date.available2019-10-06T16:11:03Z
dc.date.issued2019-03-20
dc.description.abstractIndividuals carrying the ATC/TTC haplotype (Hap-1) in the interleukin 8 (IL8) gene were reported as more susceptible to chronic periodontitis (CP), an infectious disease associated with Gram-negative bacteria, in comparison to patients with the ATT/TTC haplotype (Hap-2). This study investigated the functionality of the IL8 haplotypes in lymphocytes and monocytes of individuals carrying the Hap-1 or Hap-2 IL8 haplotypes in the response to CP-associated Gram-negative bacteria (periodontopathogens). Peripheral blood was collected from 6 subjects carrying each haplotype, and their immune cells were challenged with periodontopathogens or phorbol 12-myristate 13-acetate (PMA) plus Ionomycin. Depending on the immune cell type (lymphocytes or monocyte-derived macrophages) the assessed outcomes were: phenotypical polarization, gene expression, phagocytic activity, chemotaxis and production of reactive oxygen species (ROS). Subjects carrying the Hap-1 haplotype showed increased expression of IL8 and TNFA and significantly skewing towards pro-inflammatory Th1/M1/Th17 phenotypes. There was increased percentage of ROS-producing monocyte-derived macrophages from individuals carrying the Hap-1 haplotype. Cells from individuals presenting the Hap-2 haplotype had an overall attenuated response to periodontopathogens, with a significant shift towards the Treg phenotype. In conclusion, the IL8 haplotypes showed to be functional both in monocyte-derived macrophages and lymphocytes. The Hap-1 haplotype previously associated with increased susceptibility to CP demonstrated greater skewing to pro-inflammatory Th1/M1/Th17 phenotypes and production of ROS.en
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry at Araraquara UNESP- São Paulo State University
dc.description.affiliationDepartment of Microbiology Institute of Biomedical Sciences University of São Paulo
dc.description.affiliationDepartment of Morphology School of Dentistry at Araraquara UNESP- São Paulo State University
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry at Araraquara UNESP- São Paulo State University
dc.description.affiliationUnespDepartment of Morphology School of Dentistry at Araraquara UNESP- São Paulo State University
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 141787/2014-7
dc.format.extent152-160
dc.identifierhttp://dx.doi.org/10.1016/j.gene.2018.12.012
dc.identifier.citationGene, v. 689, p. 152-160.
dc.identifier.doi10.1016/j.gene.2018.12.012
dc.identifier.issn1879-0038
dc.identifier.issn0378-1119
dc.identifier.scopus2-s2.0-85058836472
dc.identifier.urihttp://hdl.handle.net/11449/188526
dc.language.isoeng
dc.relation.ispartofGene
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectChemokines
dc.subjectCytokines
dc.subjectInflammation
dc.subjectMacrophage
dc.subjectTh1/Th2 cells
dc.titleFunctionality of the Interleukin 8 haplotypes in lymphocytes and macrophages in response to gram-negative periodontopathogensen
dc.typeArtigo
unesp.author.lattes7634063102292261[6]
unesp.author.orcid0000-0003-1705-5481[6]

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