Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study

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dc.contributor.authorGomes, Brenda P.F.A.
dc.contributor.authorLouzada, Lidiane M.
dc.contributor.authorAlmeida-Gomes, Rebecca F.
dc.contributor.authorPinheiro, Ericka T.
dc.contributor.authorSousa, Ezilmara L.R.
dc.contributor.authorJacinto, Rogério C. [UNESP]
dc.contributor.authorArruda-Vasconcelos, Rodrigo
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversity Center Lusíada - UNILUS
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:15:29Z
dc.date.available2020-12-12T02:15:29Z
dc.date.issued2020-10-01
dc.description.abstractObjective: Identification of specific bacteria in root canals (RCs) in distinct clinical conditions can support the comprehension of pathological processes. Thus, the objective of this clinical study was to investigate the presence of F. alocis in RCs of teeth with primary endodontic infection (PEI) and with persistent/secondary endodontic infection (SEI) by using molecular techniques. It was also aimed to associate its presence with the clinical features. In addition, the levels of F. alocis as well as the total bacterial cells in the samples were also quantitated. Design: One hundred teeth (50 PEI and 50 SEI) were included. Microbial samples were performed using sterile paper points and assessed by using nested PCR and quantitative Real Time PCR (qPCR). The prevalence of F.alocis in RCs from PEI and SEI were compared by chi-square analysis. Fisher´s exact test or Pearson Chi-square, when appropriate, was used to test associations between clinical and radiographic features and the presence of F. alocis. Significance level was set at 5%. Results: F. alocis was detected in 23 and 28 (PEI) and 12 and 11 (SEI) RCs using Nested PCR and qPCR, respectively. Statistically significant associations were found between the presence of F. alocis and PEI, pain, wet canals, swelling, abscess and purulent exudate (P < 0.05). Total bacterial count was similar in both conditions (P > 0.05). Conclusions: PEI harbour a significantly higher number of F. alocis than those with SEI. Filifactor alocis was significantly associated with clinical features in primary endodontic infections. Total bacterial count was similar in both clinical conditions.en
dc.description.affiliationDepartment of Restorative Dentistry Division of Endodontics Piracicaba Dental School State University of Campinas – UNICAMP
dc.description.affiliationFaculty of Medical Sciences University Center Lusíada - UNILUS
dc.description.affiliationDepartment of Operative Dentistry Division of Endodontics The University of São Paulo School of Dentistry - FOUSP
dc.description.affiliationDepartment of Semiology and Clinic Division of Endodontics School of Dentistry Federal University of Pelotas - UFPEL
dc.description.affiliationDepartment of Endodontics Araçatuba School of Dentistry São Paulo State University - UNESP
dc.description.affiliationUnespDepartment of Endodontics Araçatuba School of Dentistry São Paulo State University - UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/23479-5
dc.description.sponsorshipIdFAPESP: 2017/25242-8
dc.description.sponsorshipIdFAPESP: 2019/19300-0
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2020.104826
dc.identifier.citationArchives of Oral Biology, v. 118.
dc.identifier.doi10.1016/j.archoralbio.2020.104826
dc.identifier.issn1879-1506
dc.identifier.issn0003-9969
dc.identifier.lattes3953630077047017
dc.identifier.orcid0000-0002-2362-8920
dc.identifier.scopus2-s2.0-85088102218
dc.identifier.urihttp://hdl.handle.net/11449/200767
dc.language.isoeng
dc.relation.ispartofArchives of Oral Biology
dc.sourceScopus
dc.subjectBacteria
dc.subjectEndodontics
dc.subjectFilifactor alocis
dc.subjectPersistent/secondary endodontic infection
dc.subjectPrimary endodontic infection
dc.subjectqPCR
dc.titleInvestigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular studyen
dc.typeArtigo
unesp.author.lattes3953630077047017[6]
unesp.author.orcid0000-0002-2362-8920[6]

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Artigos - Odontologia Restauradora - FOA